Reference

Optimized separation of pharmacologically active flavonoids from Epimedium species by capillary electrophoresis

Optimized separation of pharmacologically active flavonoids from Epimedium species by capillary electrophoresis. Liang, H. R.; Siren, H.; Riekkola, M. L.; Vuorela, P.; Vuorela, H.; Hiltunen, R. (Dep. Chem., Univ. Helsinki, Hensinki FIN-00014, Finland). Journal of Chromatography, A, 746(1), 123-129 (English) 1996 Elsevier.In this article, certain chemicals are used. Some of their cas registry numbers are 110642-44-9 and 113558-15-9 CODEN: JCRAEY. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Sixteen pharmacol. active flavonoids and one phenylethanoid glucoside isolated from Epimedium species were analyzed by capillary electrophoresis in different buffer systems of AMPSO, CAPs, phosphate and borate. The borate system proved to be the best. After optimization and studies of the effect of borate and SDS concns., org. modifier, voltage and effective capillary length on migration of the analytes, 14 flavonoids and one phenylethanoid glucoside were baseline sepd. within 20 min in the buffer system of 20 mM borate and 48 mM SDS contg. 1 mM 1, 3-diaminopropane at pH 8.5 and at the detection wavelength of 254 nm. The two-marker (xanthene-9-carboxylic acid/meso-2,3-diphenylsuccinic acid) technique and the computer program based on MATLAB were applied to improve the repeatability of anal. and to confirm the reliability of identification between two successive compds. The reliability of identification was enhanced up to 25-fold for the analytes when the migration indexes were used with the two-marker technique. .