Detail of "11079-53-1"

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Simultaneous determination of hypericin and hyperforin in human plasma with liquid chromatography-tandem mass spectrometry

Simultaneous determination of hypericin and hyperforin in human plasma with liquid chromatography-tandem mass spectrometry. Riedel, Klaus-Dieter; Rieger, Karin; Martin-Facklam, Meret; Mikus, Gerd; Haefeli, Walter E.; Burhenne, Juergen (Department of Internal Medicine VI, Clinical Pharmacology and Pharmacoepidemiology, University of Heidelberg, Heidelberg D-69120, Germany). Journal of Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences, 813(1-2), 27-33 (English) 2004 Elsevier B.V. CODEN: JCBAAI. ISSN: 1570-0232. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A selective and sensitive method for the simultaneous detn.Chemicals with cas numbers 11079-53-1 and 548-04-9 also play role. of hypericin and hyperforin-the two main active ingredients of St. John's Wort (SJW) ext.-in human plasma depending on liq./liq.-extn. and LC/MS/MS detection has been developed, validated after specifying the stability of the photosensitive hypericin in plasma samples during light exposure and applied to samples of a patient. After extn. with Et acetate/n-hexane in the darkness, sample exts. were chromatographed isocratically within 6 min on a Kromasil RP-18 column. The analytes were detected with tandem mass spectrometry in the selected reaction monitoring mode using an electrospray ion source. The limit of quantification was 0.05 ng/mL for hypericin and 0.035 ng/mL for hyperforin. The accuracy of the method varied between 101.9 and 114.2% and the precision ranged from 4.7 to 15.4% (S.D., batch-to-batch) for both analytes. The method was linear at least between 0.05 and 10 ng/mL for hypericin and between 0.035 and 100 ng/mL for hyperforin. Using this method hypericin and hyperforin were detd. successfully in a patient over seven days following discontinuation of exposure with therapeutic doses of St. John's Wort ext. .

Structural investigations of isomeric oxidized forms of hyperforin by HPLC-NMR and HPLC-MSn

Structural investigations of isomeric oxidized forms of hyperforin by HPLC-NMR and HPLC-MSn. Wolfender, J.-L.; Verotta, L.; Belvisi, L.; Fuzzati, N.; Hostettmann, K. ( Institut de Pharmacognosie et Phytochimie, Universite de Lausanne, Lausanne CH-1015, Switz.). Phytochemical Analysis, 14(5), 290-297 (English) 2003 John Wiley & Sons Ltd. CODEN: PHANEL. ISSN: 0958-0344. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) The prenylated phloroglucinol hyperforin, thought to be an essential component for the anti-depressant activity of St. John's Wort (Hypericum perforatum), is unstable. The facile oxidative degrdn. of hyperforin poses serious problems for standardisation, and may also dramatically affect the pharmacol. activity of the exts. Hyperforin was dissolved in hexane and stored at room temp. for 3 days and yielded various closely related degrdn. products which, although difficult to isolate on the preparative scale, have been analyzed by on-flow and stop-flow HPLC-NMR and HPLC-MS/MS. From online spectroscopic data, and with the aid of complementary in-mixt. 11079-53-1 and 219793-20-1 which are cas registry numbers of substances are two of reagents here. std. NMR two-dimensional correlation expts., the different oxidized forms of hyperforin were found to be phloroglucinol derivs. in which a hydroxy-dihydrofuran ring is formed involving the enol OH at C-7 or C-9 (tautomeric form) and the prenyl chain at C-8 of the core nucleus of hyperforin. The strategy followed for the online identification of these constituents is discussed. .