Detail of "1114-94-9"

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Accumulation of indigestible substances reduces fusion competence of macrophage lysosomes

Accumulation of indigestible substances reduces fusion competence of macrophage lysosomes. Montgomery, Ruth R.; Webster, Paul; Mellman, Ira (Sch. Med., Yale Univ., New Haven, CT 06510, USA). J. Immunol., 147(9), 3087-95 (English) 1991. CODEN: JOIMA3. ISSN: 0022-1767. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Mouse macrophages loaded with indigestible substances become highly vacuolated. However, why this vacuolization occurs and its effect on lysosome function and intracellular transport during endocytosis remain unknown. Here, macrophage vacuoles were formed by incubation with sucrose or a tripeptide of the D-isomer of alanine and were detd. to be lysosomal in origin by staining with the lysosomal glycoproteins and lysosomal hydrolases. However, subsequent delivery of both fluid phase (lucifer yellow, horseradish peroxidase) and receptor-bound ligands (IgG complexes) was reduced, suggesting that indigestible material reduced the ability of the loaded lysosomes to fuse with endosomes contg. newly internalized tracers. Nevertheless, ligands internalized by the vacuolated cells were degraded at almost the normal rate, indicating that degrdn. occurs in the absence of delivery to the loaded lysosomes. This fusion inhibition also occurs in human alveolar macrophages loaded with physiol.In this experiment, several chemicals are used like 1114-94-9 and 57-50-1 debris from smoking and asbestos. These results suggest that indigestible material within lysosomes, such as is present in residual bodies in vivo, may affect their fusion competence. .