Reference

Androgen metabolism in relation to growth stimulation by a uterine cell line

Androgen metabolism in relation to growth stimulation by a uterine cell line. Stenstad, Per; Oestgaard, Kjetill; Eik-Nes, Kristen B. (Inst. Biophys., Univ. Trondheim, Trondheim, Norway). Mol. Cell. Endocrinol., 8(4), 283-9 (English) 1977. CODEN: MCEND6. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) Section cross-reference(s): 13 The metab. of radioactive testosterone (I) [58-22-0], a-dihydrotestosterone [521-18-6], 4-androstene-3b,17b-diol [1156-92-9], or 4-androstene-3a,17b-diol [1852-61-5] by human cell line NHIK 3025, derived from a carcinoma of the uterine cervix, was studied. The cells were grown in Eagle's minimal essential medium (MEM) with a steroid concn. of 10-7M for 4 days. Androgen metab. by this cell line was essentially the same as for other androgen-responsive cells. The most interesting I metabolite in this system was 4-androstene-3b,17b-diol, and the sepn. of this compd. from 4-androstene-3a,17b-diol and the 2 corresponding 5a-reduced diols is described. Since 4-androstene-3b,17b-diol was a more potent growth factor for these cells than I, the small conversion of I to 4-androstene-3b,17b-diol obsd. could be responsible for the growth stimulation by I.

Participation of a nonenzymic transformation in the biosynthesis of estrogens from androgens

Participation of a nonenzymic transformation in the biosynthesis of estrogens from androgens. Goto, Junichi; Fishman, Jack (Inst. Steroid Res., Montefiore Hosp., Bronx, N. Y., USA). Science, 195(4273), 80-1 (English) 1977. CODEN: SCIEAS. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) Section cross-reference(s): 6 When placental microsomes were incubated with 1,2-3H,4-14C-labeled androst-4-ene,3,17-dione (I) [63-05-8], androst-4-ene-3.beta.,17.beta.-diol [1156-92-9], androst-4-ene-3.beta.,17.beta.,19-triol [6771-20-6], androst-4-ene-2.beta.,3.beta.,17.beta.,19-tetrol (II) [61695-88-3], and estradiol [50-28-2] were isolated as metabolites. II was found only when 2.beta.-hydroxy-19-oxoandrost-4-ene-3,17-dione (III) [54592-52-8] was added as a carrier. Apparently, the final and rate detg. hydroxylation occurs at the 2-.beta. position, and the product rapidly and nonenzymatically collapses to an estrogen.