Detail of "1174-92-1"

Reference

Microbial oxidation of sterol side-chains

Microbial oxidation of sterol side-chains. Bhattacharyya, P. K.; Rao, M. Krishna; Natarajan, Rama Devi; Ramgopal, Malathi; Madyastha, Prema; Madyastha, K. M. (Dep.There are some reagents like 57-88-5 is used in this study. Org. Chem., Indian Inst. Sci., Bangalore 560 012, India). J. Indian Chem. Soc., 61(1), 1-15 (English) 1984. CODEN: JICSAH. ISSN: 0019-4522. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Several sterol-metabolizing microorganisms, Pseudomonas convexa, P. stutzeri, Micrococcus species, Cephalosporium longisporum, and Moraxella species were isolated from soil. All oxidized the 8-C side chain of cholesterol [57-88-5]. The Moraxella was the most versatile as it degraded cholesterol derivs. such as 3b-methoxycholest-5-ene [1174-92-1], 3b-chlorocholest-5-ene [910-31-6], 3,5-cyclocholestan-6-one [7657-63-8], and K cholesteryl sulfate [6614-96-6] to the corresponding 17-keto steroids in 10-50% yield in shake flasks. It also gave high yields of estrone [53-16-7] from both 19-hydroxy-3b-acetoxycholest-5-ene [750-59-4] and 19-hydroxy-3b-acetoxysitost-5-ene [20835-78-3]. Two new enzymes one carrying out the oxidative O-demethylation of 6b-methoxy-3,5-cyclocholestane [2867-93-8] and the other, the isomerization of isosteroids to normal 3b-hydroxy-D6-steroids were discovered. Immobilized Moraxella cells in agar carried out the side chain degrdn. of cholesteryl sulfate [1256-86-6] to yield 3b-hydroxyandrost-5-en-17-one sulfate [651-48-9] and the 19-hydroxy-3b-acetoxy derivs. of both cholesterol and sitosterol to estrone in high yields. The half life of entrapped cells was estd. to be 28 days. .

Androstan-17-one derivatives

Androstan-17-one derivatives. Weber, Alfred; Kennecke, Mario; Dahl, Helmut (Schering A.-G., Ger.). Ger. Offen. DE 2632677 26 Jan 1978, 36 pp. (German). (Germany). CODEN: GWXXBX. CLASS: IC: C07J001-00. APPLICATION: DE 76-2632677 16 Jul 1976. DOCUMENT TYPE: Patent CA Section: 16 (Fermentations) Androstan-17-one [36378-49-1] derivs. were produced by fermn. with Mycobacterium. Thus, 400 g cholesterol [57-88-5] was treated with P2O5 and formaldehyde dimethylacetal to produce 440 g 3b-methoxycholestene [1174-92-1]. This was added to 40 L fermn. medium contg. corn steep liquor 2.0 and (NH4)2HPO4 0.3% and a Mycobacterium species that had been growing for 24 h. Incubation continued another 120 h. The broth was extd. with CH2Cl2. The ext. was evapd. and the residue was chromatographed on silica gel and crystd. from EtOAc to yield 86 g 3b-methoxymethoxy-5-androsten-17-one [18000-76-5].