Reference

Comparison of the effects of gossypol, 17b-estradiol and testosterone compensation on male rat reproductive organs

Comparison of the effects of gossypol, 17b-estradiol and testosterone compensation on male rat reproductive organs. Oko, R.; Hrudka, F. (West. Coll. Vet. Med., Univ. Saskatchewan, Saskatoon, SK S7H 0W0, Can.). Biol. Reprod., 30(5), 1198-207 (English) 1984. CODEN: BIREBV. ISSN: 0006-3363. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The effects of gossypol acetic acid (GAA) [12542-36-8] were compared with those induced by estradiol (E2) [50-28-2], testosterone [58-22-0], and a combination of these steroids. GAA was administered s.c. to adult rats at doses of 25-30 mg/kg/day for 30 days, whereas steroids in polydimethylsiloxane tubing of various lengths were implanted s.c. for 330 days. GAA and E2 at the doses used had similar effects: they caused a graded atrophy of sex organs, discriminative degeneration of spermatogenic cells, impairment of Sertoli cells, decrease in serum testosterone, redn. in androgen receptor binding, and retardation in body growth. Supplementing GAA and E2 treatments with 14-cm testosterone implants had a counteracting effect on organ wt. losses: seminal vesicles recovered above, ventral prostrate within, and epididymides below control values, whereas the testes did not respond. The organs most refractory to supplementation therapy were those in which GAA and E2 were most effective in depressing androgen receptor binding. Aside from having similar antiandrogenic effects as E2 and other steroids, GAA induced a specific flagellar syndrome which testosterone therapy could not prevent, indicating that this action is hormonally independent.

Rat basophilic leukemia-1 cell possesses 12-lipoxygenase and 5-lipoxygenase activities which are specifically inhibited by gossypol acetic acid

Rat basophilic leukemia-1 cell possesses 12-lipoxygenase and 5-lipoxygenase activities which are specifically inhibited by gossypol acetic acid. Hamasaki, Yuhei; Miyazaki, Sumio; Tai, Hsin Hsiung (Dep. Pediatr., Saga Med. Coll., Saga, Japan). Arerugi, 33(12), 1040-6 (Japanese) 1984. CODEN: ARERAM. ISSN: 0021-4884. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Rat basophilic leukemia-1 cells were sonicated in 1 mM EDTA-50 mM phosphate buffer-0.9% NaCl at pH 7.4, and centrifuged at 105,000 ′ g for 1 h. The sol. fraction was incubated with 14C-labeled arachidonic acid [506-32-1] in the presence of 1 mM CaCl2, and metabolites were analyzed by HPLC, gas chromatog., and mass spectrometry, successively. 5- [80619-02-9] And 12-lipoxygenase [82391-43-3] activities of the sol. fraction estd. by radiometric assay of 14C-labeled 5-hydroxyeicosatetraenoic acid [71030-39-2] and 14C-labeled 12-hydroxyeicosatetraenoic acid [71030-37-0] after incubation, resp., were inhibited by 50% by 0.29 and 0.68 mM gossypol acetic acid [12542-36-8], resp. Cycloxygenase activity estd. by prodn. of prostaglandin D2 [41598-07-6], E2 [363-24-6] and F2a [551-11-1] was inhibited by 50% by 50.3 mM gossypol acetic acid.