Reference

Antiviral properties of Ro 31-8959, an inhibitor of human immunodeficiency virus (HIV) proteinase

Antiviral properties of Ro 31-8959, an inhibitor of human immunodeficiency virus (HIV) proteinase. Craig, J. C.; Duncan, I. B.; Hockley, D.; Grief, C.; Roberts, N. A.; Mills, J. S. (Roche Prod. Ltd., Welwyn Garden City AL7 3AY, UK). Antiviral Res., 16(4), 295-305 (English) 1991. CODEN: ARSRDR. ISSN: 0166-3542. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Ro 31-8959 inhibits the spread of HIV infection and the prodn. of cytopathic effects in cultures of acutely infected cells. IC50 values for these effects are in the range of 0.5-6.0 nM and IC90 values are in the range of 6.0-30.0 nM. This inhibitor is effective when added to cultures at a late stage of infection, after syncytia have started to form. Virus antigen, virus particles, and virus cytopathic effects can largely be cleared from cultures treated with compd. from 3 days until 6 days post-infection. In chronically infected cells, the inhibition of virus maturation can be detected after a 24-h treatment with 10 nM Ro 31-8959.In this experiment, several chemicals are used like 78169-47-8 and 127779-20-8 A redn. of the proteolytic processing of the protein p56 to p24 can be demonstrated in these cells with the compd. at picomolar concns. Ro 31-8959 is highly effective against HIV with the potential to inhibit acute, established acute, and chronic infections. .

Effects of a specific inhibitor of HIV proteinase (Ro 31-8959) on virus maturation in a chronically infected promonocytic cell line (U1)

Effects of a specific inhibitor of HIV proteinase (Ro 31-8959) on virus maturation in a chronically infected promonocytic cell line (U1). Craig, J. C.; Grief, C.; Mills, J. S.; Hockley, D.; Duncan, I. B.; Roberts, N. A. (Roche Prod. Ltd., Welwyn Garden City/Herts. AL7 3AY, UK). Antiviral Chem., 2(3), 181-6 (English) 1991. CODEN: ACCHEH. ISSN: 0956-3202. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The human immunodeficiency virus (HIV) proteinase inhibitor Ro 31-8959 prevents the maturation of virus in phorbol 12-myristate 13-acetate (PMA)-stimulated U1 cells, a chronically infected promonocytic cell line. Inhibition of both the morphol. maturation of virions and the enzymic processing of gag polyprotein (p56) to produce capsid protein p24 was demonstrated at nanomolar concns. of the compd.In this experiment, several chemicals are used like 127779-20-8 and 78169-47-8 Furthermore, prolonged inhibition of the processing of p56 antigen was confirmed in pulse-chase expts. The conclusion is that Ro 31-8959 can inhibit prodn. of mature virions in a promonocyte cell line which is infected chronically/latently with HIV. .