Detail of "128-20-1"

Reference

Differential action of progesterones on hepatic microsomal activities in the rat

Differential action of progesterones on hepatic microsomal activities in the rat. Feuer, George; Kardish, R.; Farkas, R. (Banting Inst., Univ. Toronto, Toronto, Ont., Can.). Biochem. Pharmacol., 26(16), 1495-9 (English) 1977. CODEN: BCPCA6. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) Injection of rats with 5.alpha.-pregnane-3.beta.-ol-20-one (I) [516-55-2], 5.beta.-pregnane-3.alpha.-ol-20-one (II) [128-20-1], 5.alpha.-pregnane-3.beta.,20.beta.-diol (III) [516-53-0], and 5.beta.-pregnane-3.alpha.,20.alpha.-diol (IV) [80-92-2] (20 mg/kg, s.c., daily for 7 days) reduced the activities of aminopyrine N-demethylase [9037-69-8], coumarin 3-hydroxylase [9045-41-4], and glucose 6-phosphatase [9001-39-2] in hepatic microsomes. 16.alpha.-Hydroxyprogesterone (V) [438-07-3] and pregnenolone-16.alpha.-carbonitrile (VI) [1434-54-4] (20 mg/kg, s.c., daily for 7 days) increased drug metab. and glucose 6-phosphatase activity. I, II, III, and IV decreased the synthesis of microsomal phospholipids, and reduced the activity of methyltransferase [9033-25-4] and the total microsomal phosphatidylcholine (PC):phosphatidylethanolamine (PE) ratio. V and VI increased microsomal phospholipid synthesis, methyltransferase activity, and the PC:PE ratio. I, II, III, and IV decreased microsomal progesterone [57-83-0] hydroxylation in the 16.alpha.- and 6.beta.-position whereas V and VI increased it. Thus induction of the drug-metabolizing activity of the hepatic endoplasmic reticulum might be controlled by a balance in the synthesis and metab. of various progesterone derivs.

In vitro induction of nuclear breakdown in oocytes of the zebrafish, Brachydanio rerio (Ham

In vitro induction of nuclear breakdown in oocytes of the zebrafish, Brachydanio rerio (Ham. Buch.). Effects of the composition of the medium and of protein and steroid hormones. Van Ree, Goverdina E.; Lok, Dirk; Bosman, Guilielmus J. C. G. M. (Sect. Comp. Endocrinol., Zool. Lab., Utrecht, Neth.). Proc. K. Ned. Akad. Wet., Ser. C, 80(5), 353-71 (English) 1977. CODEN: KNWCAD. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) Section cross-reference(s): 12 The effectiveness of various mammalian hypophyseal hormones, fish gonadotropins, zebrafish pituitary homogenate, gonadal and adrenocortical hormones, and some of their precursors and metabolites for stimulating in vitro germinal vesicle breakdown (GVBD) in oocytes from B. rerio was studied. With the exception of zebrafish pituitary homogenate (1 pituitary/0.5 mL medium), which gave a pos. response after 1 h exposure, all tested mammalian hypophyseal hormones and fish gonadotropins (carp and salmon hypophyseal gonadotropin) failed to stimulate GVBD. Of the steroids tested in concns. of 1.3 ′ 10-6M and with incubation times from 0-24 h, progesterone, estradiol, and 11-ketotestosterone were ineffective, whereas 17a-hydroxyprogesterone [68-96-2], 20b-dihydroprogesterone [145-15-3], 17a-hydroxy-20b-dihydroprogesterone [1662-06-2], epipregnanolone [128-20-1], testosterone [58-22-0], and hydrocortisone (I) [50-23-7] were effective in stimulating GVBD after 3 h, estrone [53-16-7] after 1 h, and 11-deoxycorticosterone acetate (II) [56-47-3] after 20 min. Estrone, 17a-hydroxy-20b-dihydroprogesterone, and II were also effective in concns. from 1.3 ′ 10-7-1.3 ′ 10-5M for 3 h. A synergistic action was obsd. between II (1.3 ′ 10-6M) and zebrafish pituitary homogenate in stimulating GVBD. Moreover, pretreatment of the follicles with zebrafish pituitary homogenate enhanced the GVBD capacity of II. Apparently, one (or more) pituitary factor(s) stimulate(s) the synthesis of a deoxycorticosterone-like compd. or a metabolite which in turn initiates the maturation of oocytes.