Detail of "130100-20-8"

Reference

Fluorescence lifetime characterization of magnesium probes: Improvement of Mg2+ dynamic range and sensitivity using phase-modulation fluorometry

Fluorescence lifetime characterization of magnesium probes: Improvement of Mg2+ dynamic range and sensitivity using phase-modulation fluorometry. Szmacinski, Henryk; Lakowicz, Joseph R. (School Medicine, University Maryland, Baltimore, MD 21201, USA). Journal of Fluorescence, 6(2), 83-95 (English) 1996 Plenum. CODEN: JOFLEN. ISSN: 1053-0509. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) We measured the Mg2+-dependent absorption spectra, emission spectra, quantum yields, and intensity decays of most presently available fluorescent magnesium probes. The lifetimes were found to be strongly Mg2+ dependent for Mag-quin-1, Mag-quin-2, magnesium green, and magnesium orange and increased 2- to 10-fold upon binding of Mg2+. The lifetimes of Mag-fura-2, Mag-fura-5, Mag-fura red, and Mag-indo-1 were similar in the presence and absence of Mg2+. Detailed time-resolved measurements were carried out for Mag-quin-2 and magnesium green using phase-modulation fluorometry. Apparent dissocn. consts. (Kd) were detd. from the steady-state and time-resolved data. Their values were compared and discussed. Mg2+ sensing is described using phase and modulation data measured at a single modulation frequency. Phase angle and modulation data showed the possibility of obtaining a wider Mg2+-sensitive range than available from intensity measurements. 130100-20-8 and 184227-98-3 which are cas registry numbers of chemicals are mentioned. A significant expansion in the Mg2+-sensitive range was found for Mag-quin-2 using excitation wavelengths from 343 to 375 nm, where the apparent Kd from the phase angle was found to vary from 0.3 to about 100 mM. Discrimination against Ca2+ was also measured for Mag-quin-2 and magnesium green. Significant phototransformation and/or photodecompn., which affect the sensitivity to Mg2+, were obsd. for Mag-quin-2 and magnesium green under intense and long illumination. .