Detail of "13058-04-3"

Reference

Fermentative production of coenzyme Q10

Fermentative production of coenzyme Q10. (Daikin Kogyo Co., Ltd., Japan). Jpn. Kokai Tokkyo Koho JP 59055192 A2 30 Mar 1984 Showa, 3 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12P007-66. ICI: C12P007-66, C12R001-05. APPLICATION: JP 82-167439 24 Sep 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Isopentenyl pyrophosphate (I) [358-71-4] and farnesyl pyrophosphate (II) [13058-04-3] improved CoQ10 [303-98-0] prodn. by Alcaligenes species DK-605 or DK-515. Thus, a preculture of Alcaligenes DK-605 was inoculated into 100 mL pH 7 medium contg. glycerol 5, KH2PO4 2.1, Na2HPO4.12H2O 12, MgSO47H2O 0.3, Na2SO4 0.5, NH4Cl 1 g/L, I 0.8 and II 0.6 mM and incubated at 30° for 72 h with shaking. The broth contained 2.4 mg CoQ10/g solids, compared to 2.0 mg/g in media lacking I and II.

Microbial production of isoprenoids

Microbial production of isoprenoids. (Daikin Kogyo Co., Ltd., Japan). Jpn. Kokai Tokkyo Koho JP 59055193 A2 30 Mar 1984 Showa, 3 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12P007-66. ICI: C12P007-66, C12R001-19. APPLICATION: JP 82-167440 24 Sep 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Ubiquinone-8 (I) [2394-68-5] and (or) menaquinone-8 (II) [523-38-6] were produced from cultures of Escherichia coli that had been freeze-dried and then soaked in an aq. soln. contg. isopentenyl pyrophosphate (III) [358-71-4] and, optionally, farnesyl pyrophosphate (IV) [13058-04-3]. Thus, a preculture of E. coli K-20 W-3110 was cultivated at 37° for 7 h with shaking in 5 mL medium contg. Bacto-tryptone 10, Bacto-yeast ext. 5, NaCl 5, and glucose 1 g/L. The broth was centrifuged and the cells washed with 0.05M phosphate buffer (pH 7) and suspended in the same buffer contg. 3% Na glutamate (50 mg dry wt./mL). The suspension (0.2 mL) was freeze-dried and the freeze-dried cells were soaked in 0.1 mL 0.05M phosphate buffer contg. [14C]III (330,000 cpm) and shaken at 30° for 20 min. The cells were collected, washed, and suspended in 4 mL 0.1M phosphate buffer. The buffer soln. (67,000 cpm) was shaken with 14 mL 1:1 CHCl3-MeOH to ext. I and II into the CHCl3 layer. When the freeze-dried cells were soaked in 0.1 mL buffer contg. [14C]III (330,000 cpm) and 300 mM IV, the corresponding counting rate was 226,000 cpm.