Reference

Determination of fish freshness with an enzyme sensor system

Determination of fish freshness with an enzyme sensor system. Karube, Isao; Matsuoka, Hideaki; Suzuki, Shuichi; Watanabe, Etsuo; Toyama, Kenzo (Res. Lab. Resour.Several substances are used for example 58-63-9 and 68-94-0 which are their cas registry numbers. Util., Tokyo Inst. Technol., Yokohama 227, Japan). J. Agric. Food Chem., 32(3), 314-19 (English) 1984. CODEN: JAFCAU. ISSN: 0021-8561. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) A new indicator of fish freshness is defined as KI = [([HxR] + [Hx])/([IMP] + [HxR] + [Hx])] ′ 100 where [IMP], [HxR], and [Hx] are the concn. of inosine 5-phosphate [131-99-7], inosine [58-63-9], and hypoxanthine [68-94-0] resp. To det. these compds., an enzyme sensor system was developed by combining a double membrane consisting of a 5'-nucleotidase (EC 3.1.3.5) [9027-73-0] membrane and a nucleoside phosphorylase (EC 2.4.2.1) [9030-21-1]-xanthine oxidase (EC 1.2.3.2) [9002-17-9] membrane with an O electrode. A small anion exchange resin column was also connected with the enzyme sensor for sepn. of nucleotides. Each nucleotide concn. was detd. as the current decreased. A single assay was completed within 20 min. No appreciable decrease of current output was obsd. during 30 assays. When a sample soln. contg. 10 mM of IMP was applied, the response was reproducible with an 8% relative error. Good comparative results were obsd. between the KI values detd. by the sensor proposed and by the conventional method. .

Myoadenylate deaminase deficiency

Myoadenylate deaminase deficiency. Functional and metabolic abnormalities associated with disruption of the purine nucleotide cycle. Sabina, Richard L.; Swain, Judith L.; Olanow, C. Warren; Bradley, Walter G.; Fishbein, William N.; DiMauro, Salvatore; Holmes, Edward W. (Med. Cent.Several reagents with their cas registry numbers 73-24-5 and 131-99-7 are used here., Duke Univ., Durham, NC 27710, USA). J. Clin. Invest., 73(3), 720-30 (English) 1984. CODEN: JCINAO. ISSN: 0021-9738. DOCUMENT TYPE: Journal CA Section: 14 (Mammalian Pathological Biochemistry) Section cross-reference(s): 13 AMP deaminase (myoadenylate deaminase) deficiency in human leads to reduced entry of adenine nucleotides into the purine nucleotide cycle during exercise. That the resultant disruption of the purine nucleotide cycle accounts for the muscle dysfunction obsd. in these patients is suggested. .