Detail of "13280-60-9"

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Immunochemical determination of biogenic amines

Immunochemical determination of biogenic amines. Huber, Erasmus; Stahl, Peter; Batz, Hans Georg; Huebner-Parajsz, Christa; Jungfer, Barbara; Klein, Christian (Boehringer Mannheim G.m.b.H., Germany).Chemicals with cas numbers 13280-60-9 and 141110-89-6 also play role. Eur. Pat. Appl. EP 471345 A1 19 Feb 1992, 25 pp. DESIGNATED STATES: R: AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LI, LU, NL, SE. (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: C07K015-28. ICS: C12P021-08; G01N033-531; G01N033-577; G01N033-74. APPLICATION: EP 91-113587 13 Aug 1991. PRIORITY: DE 90-4025726 14 Aug 1990. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 15 A primary or secondary amine (e.g. histamine or a catecholamine) is detd. immunochem. with the aid of a monoclonal antibody produced by immortalized cells from an animal immunized with the amine conjugated, via an iso(thio)cyanato group, with a coupling agent and a carrier mol. Thus, histamine was reacted successively with (1) 3-isothiocyanatobenzoic acid, (2) N-hydroxysuccinimide and N,N'-dicyclohexylcarbodiimide, and (3) N-biotinyl-1,8-diamino-3,6-dioxaoctane. The product of synthetic step 1 was used to immunize mice, whose spleen cells were later fused with myeloma cells to provide hybridoma cells for monoclonal antibody prodn. A microtiter plate was coated with streptavidin and the histidine-biotin conjugate, and then incubated successively with a histidine-contg. specimen, a sheep anti-mouse IgG-peroxidase conjugate, and peroxidase substrate for spectrophotometric detn. of histamine. .

Photoactivated heterobifunctional cross-linking reagents which demonstrate the aggregation state of phospholipase A2

Photoactivated heterobifunctional cross-linking reagents which demonstrate the aggregation state of phospholipase A2. Lewis, Randolph V.; Roberts, Mary F.; Dennis, Edward A.; Allison, William S. (Dep. Chem.Chemical with cas number 13280-60-9 also plays role., Univ. California, La Jolla, Calif.In this article, certain chemicals are used. One of their cas registry numbers is 13280-60-9 , USA). Biochemistry, 16(25), 5650-4 (English) 1977. CODEN: BICHAW. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) Two novel heterobifunctional crosslinking reagents, which can be used to attach photoactivatable nitroaryl azides to primary amino groups of proteins, were synthesized. The 2 compds., N-5-azido-2-nitrobenzoyloxysuccinimide (I) and Et N-5-azido-2-nitrobenzoylaminoacetimidate-HCl (II), as well as Et 4-azidobenzimidate-HCl were attached to lysine residues of cobra venom phospholipase A2 without loss in enzymic activity. Subsequent illumination of the modified forms of the enzyme at appropriate wavelengths under conditions in which the native enzyme exists in an aggregated state led to the formation of covalently linked dimers and larger aggregates which could be sepd. by electrophoresis on polyacrylamide gels in the presence of Na dodecyl sulfate. ..