Reference

Determination of a novel anti-psychotic agent AD-5423 and its metabolites in plasma by high-performance liquid chromatography with fluorescence detection

Determination of a novel anti-psychotic agent AD-5423 and its metabolites in plasma by high-performance liquid chromatography with fluorescence detection. Matsuda, Michiaki; Sakashita, Motoko; Yamaguchi, Toshikazu; Fujii, Toshihiko (Department of Pharmacokinetics, Developmental Research Laboratories, Dainippon Pharmaceutical Co. Ltd., Suita 564, Japan). Journal of Pharmaceutical and Biomedical Analysis, 15(9,10), 1449-1456 (English) 1997 Elsevier. CODEN: JPBADA. ISSN: 0731-7085. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) AD-5423, 2-(4-ethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocyclo octa[b]pyridine, is a novel antipsychotic agent. To investigate the pharmacokinetics of AD-5423, a simple and sensitive high-performance liq. chromatog. (HPLC) method has been developed for the simultaneous detn. of AD-5423 and its two N-oxidized metabolites (N-desethyl AD-5423 and AD-5423 N-oxide) in plasma. After pretreatment of a plasma sample by solid-phase extn., AD-5423 and its metabolites were analyzed on a HPLC with fluorescence detection (335/410 nm). Chromatog. was performed on two C18 reversed-phase columns connected by a switching system, with a mobile phase of acetonitrile-methanol-25 mM sodium phosphate buffer (pH 2.5) contg. 25 mM sodium 1-heptanesulfonate (:38, vol./vol./v). The method gives satisfactory accuracy and precision for the detn. of AD-5423 and its metabolites. In human plasma, accurate detns. are possible over the concn. ranges of 0.04-5 ng ml-1 for AD-5423 and 0.1-5 ng ml-1 for N-oxidized metabolites. The intra- and inter-day assay precision (R.S.D.) of AD-5423 (0.5 ng ml-1) were 3.6 and 7.Some chemicals with cas registry numbers like 132810-10-7 are also used.2%, resp.In this experiment, several chemicals are used like 132810-10-7 In plasma of exptl. animals, the validated quant. range are 0.1-100 ng ml-1 for both AD-5423 and its metabolites. ..

Determination of a novel anti-psychotic agent AD-5423 and its metabolites in plasma by high-performance liquid chromatography with fluorescence detection

Determination of a novel anti-psychotic agent AD-5423 and its metabolites in plasma by high-performance liquid chromatography with fluorescence detection. Matsuda, Michiaki; Sakashita, Motoko; Yamaguchi, Toshikazu; Fujii, Toshihiko (Department of Pharmacokinetics, Developmental Research Laboratories, Dainippon Pharmaceutical Co. Ltd., Suita 564, Japan). Journal of Pharmaceutical and Biomedical Analysis, 15(9,10), 1449-1456 (English) 1997 Elsevier. CODEN: JPBADA. ISSN: 0731-7085. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) AD-5423, 2-(4-ethyl-1-piperazinyl)-4-(4-fluorophenyl)-5,6,7,8,9,10-hexahydrocyclo octa[b]pyridine, is a novel antipsychotic agent. To investigate the pharmacokinetics of AD-5423, a simple and sensitive high-performance liq. chromatog. (HPLC) method has been developed for the simultaneous detn. of AD-5423 and its two N-oxidized metabolites (N-desethyl AD-5423 and AD-5423 N-oxide) in plasma. After pretreatment of a plasma sample by solid-phase extn., AD-5423 and its metabolites were analyzed on a HPLC with fluorescence detection (335/410 nm). Chromatog. was performed on two C18 reversed-phase columns connected by a switching system, with a mobile phase of acetonitrile-methanol-25 mM sodium phosphate buffer (pH 2.5) contg. 25 mM sodium 1-heptanesulfonate (:38, vol./vol./v). The method gives satisfactory accuracy and precision for the detn. of AD-5423 and its metabolites. In human plasma, accurate detns. are possible over the concn. ranges of 0.04-5 ng ml-1 for AD-5423 and 0.1-5 ng ml-1 for N-oxidized metabolites. The intra- and inter-day assay precision (R.S.D.) of AD-5423 (0.5 ng ml-1) were 3.6 and 7.Some chemicals with cas registry numbers like 132810-10-7 are also used.2%, resp.In this experiment, several chemicals are used like 132810-10-7 In plasma of exptl. animals, the validated quant. range are 0.1-100 ng ml-1 for both AD-5423 and its metabolites. ..