Detail of "133-89-1"

Reference

Characteristics of nucleotide and nucleic acid metabolism in oxythiamin-induced vitamin B1 deficiency

Characteristics of nucleotide and nucleic acid metabolism in oxythiamin-induced vitamin B1 deficiency. Khokha, A. M.; Fustochenko, B. P.; Ostrovskii, Yu. M. (Div. Metab. Regul., Grodno, USSR). Biokhimiya (Moscow), 49(3), 361-9 (Russian) 1984. CODEN: BIOHAO.Several reagents with their cas registry numbers 56-65-5 and 65-86-1 are used here. ISSN: 0006-307X. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) In male rats made thiamin [59-43-8] deficient by oxythiamin treatment (40 mg/100 g body wt.), decreases in liver orotic acid [65-86-1] (3.32 vs. 4.59 mmol/mg DNA), UDP glucose [133-89-1] (101.2 vs. 119.3 mmol/mg DNA), ATP [56-65-5] (546.6 vs. 616.1 mmol/mg DNA), and pyrimidine [289-95-2] levels (283.4 vs. 314.6 mmol/mg DNA) were obsd., along with decreased RNA synthesis (3082 vs. 4374 units/mg RNA, as measured by 14C-orotate utilization). There was no decrease in total liver RNA, however. .

D-Glucosamine-induced changes in nucleotide metabolism and growth of colon-carcinoma cells in culture

D-Glucosamine-induced changes in nucleotide metabolism and growth of colon-carcinoma cells in culture. Krug, Eva; Zweibaum, Alain; Schulz-Holstege, Christa; Keppler, Dietrich (Unite Rech. Metab. Differ. Cell. Cult., Hop. Broussais, Paris 75674, Fr.). Biochem. J., 217(3), 701-8 (English) 1984. CODEN: BIJOAK. ISSN: 0306-3275. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Human colon-carcinoma cells were exposed to D-glucosamine [3416-24-8] at concns. (2.5, 5 and 10 mM) that were growth-inhibitory but not cytocidal in the presence of a physiol. glucose concn. Labeling of these HT-29 cell with D-[14C]-glucosamine, followed by nucleotide analyses, demonstrated that UDP-N-acetylhexosamines represented the major intracellular nucleotide pool and the predominant metabolite of the amino sugar. D-[14C]glucosamine was not a precursor of UDP-glucosamine [17479-04-8]. After 4 h exposure to D-glucosamine (2.5 mM), the pool of UDP-N-acetylhexosamines was increased >6-fold, whereas UTP [63-39-8] and CTP [65-47-4] were markedly decreased. UDP-glucuronate [2616-64-0] content increased by >-fold, whereas purine nucleotide content was little altered. Uridine (0.1 mM) largely reversed the decrease in UTP, CTP, UDP-glucose [133-89-1] and UDP-galactose [2956-16-3], whereas the UDP-N-acetylhexosamine pool was increased. Uridine did not reverse the D-glucosamine-induced retardation of growth in culture. A 50% decrease in growth also persisted when uridine and cytidine, cytidine alone, or UDP, were added together with D-glucosamine. The growth-inhibitory effect of the amino sugar could therefore be best correlated with the quant. change in the pattern of sugar nucleotides, and, in particular, with the large increase in UDP-N-acetylglucosamine [528-04-1] and UDP-N-acetylgalactosamine [7277-98-7].