Detail of "13345-51-2"

Reference

Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts

Effects of bradykinin on DNA synthesis in resting NIL8 hamster cells and human fibroblasts. Straus, Daniel S.; Pang, Kenneth J. (Dep. Biol., Univ. California, Riverside, CA 92521, USA). Exp. Cell Res., 151(1), 87-95 (English) 1984. CODEN: ECREAL. ISSN: 0014-4827. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Bradykinin [58-82-2] stimulates [3H]thymidine incorporation and DNA synthesis in resting, serum-deprived NIL8 hamster cells. The ED50 for this stimulation is 4.52 nM. Other kinin peptides (including Lys-bradykinin (kallidin) [342-10-9] and Met-Lys-bradykinin [550-19-6]) also stimulate [3H]thymidine incorporation into the NIL8 cells, whereas desArg9-bradykinin [15958-92-6] is without effect, suggesting that the action of the kinin peptides is through type B2 receptors. Bradykinin also stimulates DNA synthesis in IMR-90 human fibroblasts; however, this effect is obsd. only in the presence of indomethacin, which blocks prostaglandin synthesis. Apparently, prostaglandins act as neg. modulators of the growth-stimulatory effects of bradykinin in the fibroblasts. This conclusion is supported by the observation that exogenously added PGE1 [745-65-3], PGE2 [363-24-6], PGA1 [14152-28-4], PGA2 [13345-50-1], PGB1 [13345-51-2], and PGB2 [13367-85-6] strongly inhibit [3H]thymidine incorporation in the human fibroblasts. The direct effect of bradykinin obsd. 745-65-3 and 58-82-2 are just another two chemicals used in this study. in the NIL8 cells may be attributable to the relative resistance of these cells to growth inhibition by prostaglandins. .

Quantitative determination of prostaglandins A1 and B1 in alprostadil (PGE1) by high-performance liquid chromatography

Quantitative determination of prostaglandins A1 and B1 in alprostadil (PGE1) by high-performance liquid chromatography. Zoutendam, P. H.; Bowman, P. B.; Rumph, J. L.; Ryan, T. M. (Cont.Some commonly used reagents like 745-65-3 and 14152-28-4 are used in this experiment. Anal. Res. Dev., Upjohn Co., Kalamazoo, MI 49001, USA). J. Chromatogr., 283, 281-7 (English) 1984. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Absorption high-performance liq. chromatog. was used for the detn. of PGA1 (I) [14152-28-4] and nPGB1 (II) [13345-51-2] in alprostadil [745-65-3] and in Prostin VR Pediatric Sterile Soln. I and II were the major degrdn. products of PGE1. The adsorption system provided baseline resoln. of the 2-naphthacyl esters of I from II. Derivatization conditions providing maximal response for I and minimizing the conversion of PGE1 to I and II were established. .