Detail of "135-16-0"

Reference

Enzymic synthesis of L-serine

Enzymic synthesis of L-serine. Anderson, D. M.; Hsiao, H. Y. 56-40-6 and 50-00-0 are also occured in this study.; Somerville, R. L.; Herrmann, K. M. (Genex Corp., USA). Belg. BE 898261 A1 16 Mar 1984, 21 pp. (French). (Belgium). CODEN: BEXXAL. ICI: C12; C12. APPLICATION: BE 21-898 18 Nov 1983. PRIORITY: US 82-442962 19 Nov 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) L-Serine [56-45-1] is produced from glycine [56-40-6] and HCHO [50-00-0] in the presence of tetrahydrofolate [135-16-0] by bacteria contg. serine hydroxymethyltransferase [9029-83-8]. Thus, a culture of Escherichia coli GX1703 was centrifuged to recover the cells, which were suspended in a mixt. of glycine 12, tetrahydrofolate 5, pyridoxal phosphate 1, and HCHO 10 mM in pH 7.6 buffer under N2. The mixt. was stirred at 37° for 8 h. The concn. of serine was 5 mM (45% yield). .

Fluorometric determination of folacin in biological materials using high performance liquid chromatography

Fluorometric determination of folacin in biological materials using high performance liquid chromatography. Gregory, Jesse F., III; Sartain, Doris B.; Day, Brian P. F. (Food Sci. Human Nutr. 4033-27-6 and 58-05-9 are also occured in this study. Dep., Univ. Florida, Gainesville, FL 32611, USA). J. Nutr., 114(2), 341-53 (English) 1984. CODEN: JONUAI. ISSN: 0022-3166. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) Section cross-reference(s): 9 A procedure was developed for the quantitation of the major folacin [59-30-3] compds. present in foods and other biol. materials. Extn. conditions were selected to provide conversion of 10-formyltetrahydrofolic acid to 5-formyltetrahydrofolic acid [58-05-9]. Polyglutamyl folates were deconjugated with hog kidney conjugase. The resulting folacin monoglutamates were sepd. by reverse-phase high-performance liq. chromatog. after extn. purifn. on an anion exchange column. Detection of tetrahydrofolic acid [135-16-0] and its substituted derivs. was performed by monitoring the native fluorescence of the reduced folates in the acidic mobile phase. Folic acid, dihydrofolic acid [4033-27-6], and also tetrahydrofolic acid were measured fluorometrically by using an oxidative postcolumn derivatization system in series with the 1st fluorometer. Detection limits ranged from 0.03 to 2.3 pmol/100 mL injection for the various folates. The validity of the method was supported by recovery and fluorescence spectral studies and by comparison with Lactobacillus casei assays for a variety of samples. Anal. of rat liver following a single pulse dose of 3H-labeled folic acid indicated large differences between the patterns of radiolabeled and endogenous folates. .