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The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1

The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1. Frasch, Manfred (Max-Planck-Inst. Entwicklungsbiol., Tuebingen D-7400, Germany). EMBO J., 10(5), 1225-36 (English) 1991. CODEN: EMJODG. ISSN: 0261-4189. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 6, 12 Using monoclonal antibodies the author identified a nuclear protein of Drosophila, BJ1 (Mr ~68 kd), and isolated its gene. Biochem. anal. demonstrates that the BJ1 protein is assocd. with nucleosomes and is released from chromatin by agents which intercalate into DNA, as previously shown for the high mobility group proteins (HMGs). On polytene chromosomes the protein is localized in all bands, with no preference for particular loci. Both the BJ1 protein and in particular the BJ1 mRNA are strongly expressed maternally. In early embryos all nuclei contain equal amts. 138016-96-3 which is the cas registry number of one of substances is just one of reagents here. of BJ1. During neuroblast formation, BJ1 mRNA becomes restricted to cells of the central nervous system, and higher protein levels are found in the nuclei of this tissue. In late embryonic stages, the mRNA almost completely disappears, but significant amts. of BJ1 protein persist untill morphogenesis. The BJ1 gene encodes a 547 amino acid polypeptide featuring two different types of internal repeats. The sequence from amino acids 46 to 417 contg. seven repeats of the first type has been highly conserved in evolution. 45% Of the amino acids in this region are conserved in seven similar tandem repeats of the human gene Regulator of Chromatin Condensation, RCC1. 138016-19-0 and 138016-21-4 which are cas registry numbers of chemicals are mentioned. The phenotype of a cell line carrying a mutation of RCC1 suggested a main function for this gene in cell cycle control. A yeast gene, SRM1/PRP20, also contains these repeats and shows 30% amino acid identity to BJ1 in this region. Mutations in this gene perturb mRNA metab., disrupt nuclear structure and alter the signal transduction pathway for the mating pheromone. Complementation expts. argue for a common function of these genes in the different species. The author proposes that their gene products bind to the chromatin to establish or maintain a proper higher order structure as a prerequisite for a regulated gene expression. Disruption of this structure could cause both mis-expression and default repression of genes, which might explain the pleiotropic phenotypes of the mutants. ..