Detail of "139569-17-8"

Reference

Nucleotide sequence of the Spiroplasma citri fibril protein gene

Nucleotide sequence of the Spiroplasma citri fibril protein gene. Williamson, David L.; Renaudin, Joel; Bove, Joseph M. (Health Sci. Cent., State Univ. New York, Stony Brook, NY 11794-8081, USA). J. Bacteriol., 173(14), 4353-62 (English) 1991. CODEN: JOBAAY. ISSN: 0021-9193. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 6 Electron microscopic observation of spiroplasms lysed by detergent (sodium deoxycholate) revealed the release of bundles of fibrils from the cells. Individual fibrils are 4 nm in diam. and possess a 9-nm periodicity along their length. These fibrils are thought to function as cytoskeletal structures involved in the shape and motility of spiroplasmas. Polyacrylamide gel electrophoresis of d. gradient-purified fibrils showed a protein of approx. 55 kDa. Oligonucleotide probes were constructed from the N-terminal amino acid sequence of two peptides obtained after V8 protease hydrolysis of the fibril protein. The probes were used to identify the clones in a genomic DNA library of S. citri that contained inserts carrying the probe sequence. Sequencing of a 3.3-kbp fragment yielded the full open reading frame of the fibril protein gene and the start of a second open reading frame of an unknown protein. The fibril protein is composed of 515 amino acids, which have a computed mol. mass of 59 kDa. Northern (RNA) blot hybridization and primer extension expts. showed that transcription of the fibril protein gene starts from a promoter located 100 nucleotides upstream of the initiation codon and stops at a rho-independent type terminator, leading to a 1.7-kbp transcript. Southern blot hybridization of genomic DNA using the fibril protein ge ne as the probe showed that a single copy of the gene is present in the chromosomes of both S. citri and S. 139570-20-0 and 139569-17-8 are just another two chemicals used in this study. melliferum. The genotypic symbol fib is proposed for the spiroplasma fibril protein gene. .