Detail of "139664-41-8"

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Deguelin cyclase, a prenyl to chromen transforming enzyme from Tephrosia vogellii

Deguelin cyclase, a prenyl to chromen transforming enzyme from Tephrosia vogellii.Some chemicals with cas registry numbers like 7782-44-7 and 139664-41-8 are also used. Crombie, Leslie; Rossiter, John T.; Van Bruggen, Nicholas; Whiting, Donald A. (Dep. Chem., Univ. Nottingham, Nottingham NG7 2RD, UK). Phytochemistry, 31(2), 451-61 (English) 1992. CODEN: PYTCAS. ISSN: 0031-9422. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) Section cross-reference(s): 11 Seeds and plant parts of T. vogellii were investigated in order to provide systems for the study of the prenyl-to-chromen transformation in rotenoids, as exemplified by the conversion of rot-2-enonic acid into deguelin. No hydroxylated intermediate was found. A cell-free prepn. was obtained from T. vogellii seedlings or seeds and shown to catalyze the reaction. The water-sol. enzyme was partially purified using (NH4)2SO4 pptn., gel chromatog., and ion-exchange procedures. Data for the enzyme, named deguelin cyclase (I), are reported, including optima for pH and temp. and Km values (which indicates strong binding between enzyme and substrate). The results relevant to mol. wt. detn. were discussed. I had a requirement for O2, but not for cofactors. I was inhibited by Cl- and chelating agents, esp. 1,10-phenanthroline. I could convert the 11-hydroxyrotenoid, sumatrolic acid, into a-toxicarol, and lapachol into dehydro-a-lapachone, although the prenyl-to-chromen conversion was not general. I did not convert rot-2'-enonic acid into rotenone under the conditions studied. 139664-41-8 is also in the experiment. I appeared not to belong to the cytochrome P 450 group and resembled more closely the nonheme Fe enzyme, isopenicillin N synthase. ..