Reference

Antibiotics and bacterial auxotrophic mutants in the cocultivation of plant protoplasts and bacterial cells or spheroplasts

Antibiotics and bacterial auxotrophic mutants in the cocultivation of plant protoplasts and bacterial cells or spheroplasts. Christen, Alice A.; Kirkpatrick, Mary Ann; Lurquin, Paul F. (Program Genet. Cell Biol., Washington State Univ., Pullman, WA 99164-4350, USA). Z. Pflanzenphysiol., 113(3), 213-21 (English) 1984. CODEN: ZSPPAD.Several reagents with their cas registry numbers 35607-66-0 and 1404-90-6 are used here. ISSN: 0044-328X. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) The toxicity of 6 b-lactam antibiotics to Nicotiana tabacum cv. xanthi cells and protoplasts as well as their effect on Agrobacterium tumefaciens cells contg. the bla gene from pBR322 and the plasmid pPH1J1 of the P incompatibility group were studied. Relatively high doses of ampicillin, cloxacillin, cefoxitin, and vancomycin can be tolerated by plant cells while allowing rapid elimination of A. tumefaciens cells. However, young cells originating from protoplasts are more sensitive to antibiotics. A combination of cefoxitin and cloxacillin allows good control of bacterial proliferation and has no adverse effect on plant protoplast division. Histidine- and leucine-requiring mutants of A. tumefaciens proliferate very slowly in the presence of dividing protoplasts, whereas E. coli HB101 carrying a bla gene do not. A. rhizogenes Was also found to be sensitive to cefoxitin. .

Formation of copper(II)-vancomycin chelate

Formation of copper(II)-vancomycin chelate. Application to determination of the antibiotic dosage by continuous flow and amperometric detection. Chabenat, C.; Andre, D.; Boucly, P. 1404-90-6 and 7440-50-8 are also occured in this study. (Lab. Chim. Anal. Pharm., UER Med. Pharm. Rouen, St. Etienne-du-Rouvray 76800, Fr.). Talanta, 30(12), 963-6 (French) 1983. CODEN: TLNTA2. ISSN: 0039-9140. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) The formation of a metal-complex of Cu(II) with vancomycin [1404-90-6], an antibiotic active towards Gram-pos. bacteria, was proved by spectrophotometric, polarog., and potentiometric methods. Half-wave redn. potentials and voltamperograms indicate the stoichiometry of the addn. compd. and the equil. const. This complex was used for detn. of vancomycin by a continuous flow method with Cu(II) and amperometric detection in a polarog. cell of thin-layer type. .