Reference

The disturbance of oxidative phosphorylation in rat liver mitochondria by the carcinogens 12-hydroxystearic acid and its methyl ester

The disturbance of oxidative phosphorylation in rat liver mitochondria by the carcinogens 12-hydroxystearic acid and its methyl ester. Hadler, Herbert I.; Mueller, Kenneth W. (Dep. Chem. Biochem., South. Illinois Univ., Carbondale, Ill., USA). J. Environ. Pathol. Toxicol., 1(1), 75-85 (English) 1977. CODEN: JEPTDQ. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Addn. of 30mM 12-hydroxystearic acid (I) [106-14-9] or its Me ester (II) [141-23-1] into the rat liver mitochondrial prepn. caused significant damage and uncoupling of the mitochondrial function. Both I and II induced mitochondrial ATPase [9000-83-3]. In the absence of ATP, I induced a small, but significant change in the mitochondrial vol.; addn. of ATP enhanced this swelling phenomenon. II caused a small delayed mitochondrial vol. change in the presence or absence of ATP. The decrease in absorbancy of the mitochondrial prepn. at 520 nm by I and II was blocked by various inhibitors even though the mitochondria were not stabilized by bovine serum albumin indicating that the changes in the absorbance were not due to nonspecific lysing of the mitochondria and the 2 agents were not acting as nonspecific detergents. Thus, the carcinogens I and II interfere with oxidative phosphorylation in rat liver mitochondria.

Photodegradation chemistry of the insecticide phosmet in lipid models and in the presence of wool wax, employing a 15N-labeled compound

Photodegradation chemistry of the insecticide phosmet in lipid models and in the presence of wool wax, employing a 15N-labeled compound. Sinderhauf, Katrin; Schwack, Wolfgang (Institut fuer Lebensmittelchemie, Universitaet Hohenheim, Stuttgart D-70593, Germany). Journal of Agricultural and Food Chemistry, 52(26), 8046-8052 (English) 2004 American Chemical Society. CODEN: JAFCAU. ISSN: 0021-8561. DOCUMENT TYPE: Journal CA Section: 5 (Agrochemical Bioregulators) The organophosphorus insecticide phosmet is commonly used for plant protection as well as against pests on animals. Phosmet features numerous degrdn. pathways induced by UV irradn. In this study, we focused on the reaction possibilities of phosmet in the presence of lipophilic substances with olefinic structure elements, as they are frequently found in animal fur lipids, esp. in wool wax. In the first step, simple models were employed to characterize the structural types of formed photoaddn. products. On irradn. in the presence of cyclohexene, three photoaddn. products were identified, a (4p + 2p) cycloaddn. product of phosmet and cyclohexene and two diastereoisomer carbinols. Likewise, in more sophisticated models employing fatty acid Me esters for irradn. expts., phosmet was readily photodegraded. In the presence of Me oleate, also 1:1 photoaddn. products could easily be identified by means of liq. 112-62-9 and 141-23-1 are cas registry numbers of chemicals which are used as reagents here. chromatog.-mass spectrometry, using a stable isotope-labeled phosmet (15N-phosmet, 50 at. %) for the photolysis expts. To converge from models to the more complex natural environment of animal skin lipids, irradn. expts. of phosmet in the presence of wool wax were performed. After 24 h of irradn., only a remnant of 1.4% of the initial phosmet was detectable. Phosmet-oxon was detected in concns. up to 27.3 mol%. .