Reference

Effects of monosodium glutamate administration in the neonatal period on the diabetic syndrome in KK mice

Effects of monosodium glutamate administration in the neonatal period on the diabetic syndrome in KK mice. Cameron, D. P.; Poon, T. K. Y.; Smith, G. C. (Med. Res. Cent., Prince Henry's Hosp., Melbourne, Aust.). Diabetologia, 12(6), 621-6 (English) 1976. CODEN: DBTGAJ. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) Administration of monosodium glutamate (MSG) [142-47-2] to KK mice during the neonatal period resulted in a syndrome of obesity, stunting and hypogonadism. In some animals the genetic predisposition to diabetes was unmasked with the development of marked hyperglycemia and or hyperinsulinemia. Food intake was not increased compared to controls. The elevated plasma glucose and insulin [9004-10-8]in fed MSG treated mice fell rapidly with food deprivation. Glucose disposal was comparable in MSG treated and control mice ater i.p. glucose, but after oral glucose MSG treated mice showed impaired glucose tolerance. Insulin secretion was defective in MSG treated mice after i.p. but not after oral glucose.

Methods for monosodium glutamate determination

Methods for monosodium glutamate determination. II. Canned soups, sauces, cooking pans, seasonings, and various aromas. Armand, Paul; Abello, Georges; Gayte-Sorbier, Andre (Fac. Pharm., Marseille, Fr.). Ann. Falsif. Expert. Chim., 69(742), 545-56 (French) 1976. CODEN: AFECAT. DOCUMENT TYPE: Journal CA Section: 17 (Foods) The 2 methods used previously (thin-layer chromatog. and enzymic with glutamate dehydrogenase and detn. of NADH at 320 mm) (Gayte-Sorbier, A., et al., 1975) for detg. mono-Na glutamate [142-47-2] in dried soup were successfully adapted for use on the title products. In addn., the enzymic method was modified for final photometry in the visible by coupling glutamate dehydrogenase with diaphorase from pig heart and iodonitrotetrazolium chloride and detg. the color of the reduced formazan at 492 nm. All 3 methods give essentially the same results with the title foods. Ascorbic acid interfered in the colorimetric variation, forming a pink color with the tetrazolium reagent; however, the interference could be minimized by shortening the reaction time, and the detn. was possible if the ascorbate-to-glutamate ratio were <10. Solid foods were homogenized, stirred with water, centrifuged, and an aliquot of the supernatant used; liq. products were stirred, centrifuged, and an aliquot used. Glutamate was stable to normal conditions of cooking and processing of food.