Detail of "142849-53-4"

Reference

Screening of combinatorial peptide libraries for selection of peptide ligand useful in affinity purification of target proteins

Screening of combinatorial peptide libraries for selection of peptide ligand useful in affinity purification of target proteins. Buettner, Joseph A. (Bayer A.-G., USA). Eur. Pat. Appl. EP 742438 A2 13 Nov 1996, 19 pp. DESIGNATED STATES: R: AT, BE, CH, DE, DK, ES, FI, FR, GB, GR, IE, IT, LI, LU, MC, NL, PT, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: G01N033-68. ICS: G01N033-543; C07K001-04; C07K001-22. APPLICATION: EP 1996-106717 29 Apr 1996. PRIORITY: US 1995-438331 10 May 1995. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 1, 34, 66 Ligands that interact with a target can be more easily identified if false pos. interactions (either specific or nonspecific) from the detecting system are differentiated from the target-specific interaction. An improved method of identifying peptides which bind with a target protein is presented. The steps are: binding a random library of peptides to a support material (e.g., chromatog. support), allowing detection reagents (e. 150112-96-2 and 142849-53-4 are also in the experiment.g., antibodies and their enzyme conjugates) to contact the peptides and the support material and identifying these interactions, then allowing the target protein to selectively bind to the peptides, allowing detection reagents to contact the bound target protein, and characterizing the peptide bound to the identified support material. Interaction of a ligand or the support material with the detection reagents will cause a distinct color change which distinguishes those ligands which selectively bind to target protein. The characterized peptide can then be bound to a chromatog. support for use in affinity purifn. of the target protein. In one embodiment, automation of the assay is demonstrated by flowing all immunoreagents through the beads in a column format ensuring highly efficient washing. In the preferred embodiment, a resin for peptide synthesis is used that is hydrophilic, contains spacers and may exhibit less nonspecific background than other resins and permits synthesis and direct evaluation of combinatorial peptide libraries for binding to target proteins. Examples for the use of this new resin and methodol. for identifying peptide-ligands for purifn. of protein are presented. .