Reference

Effects of bile salts and prostaglandins on sodium transport in isolated rat gastric mucosa

Effects of bile salts and prostaglandins on sodium transport in isolated rat gastric mucosa. Nguyen, D. A.; Sernka, T. J. (Sch. Med., Wright State Univ., Dayton, OH 45435, USA). Gen. Physiol. Biophys., 4(4), 337-48 (English) 1985. CODEN: GPBIE2. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 13 To det. whether prostaglandins may protect against bile salt inhibition of Fe transport in the stomach, gastric mucosal tissue was isolated from the rat and mounted in flux chambers. Transport of Na+ was traced with radioisotopes in the absence of bile salts and then in the presence of conjugated Na taurocholate [145-42-6] or unconjugated Na deoxycholate [302-95-4] at low, intermediate, and high mucosal concns. (1, 5, and 15 mM). At a high (7.40) or low (3.4) mucosal pH, only the unconjugated deoxycholate inhibited active Na+ transport from mucosa to submucosa with respect to untreated controls. Inhibition of Na+ transport was apparent at a low level of deoxycholate, which also inhibited the elec. p.d. Intermediate and high levels of deoxycholate lowered the tissue resistance. When the tissues were exposed to mucosal PGE2 [363-24-6] or its 16,16-dimethyl analog [39746-25-3] before and during acidified taurocholate administration, Na+ transport was not changed significantly but the elec. resistance remained high. Thus, unconjugated bile salt is more potent than conjugated bile salt in inhibiting Na+ transport and breaking the gastric mucosal barrier, and prostaglandins may afford some small protection.

Effect of rice hemicelluloses on pancreatic lipase activity in vitro

Effect of rice hemicelluloses on pancreatic lipase activity in vitro. Normand, Floyd L.; Ory, Robert L. (South. Res. Res. Cent., USDA-ARS, New Orleans, LA 70179, USA). J. Food Sci., 49(3), 956-7 (English) 1984. CODEN: JFDSAZ. ISSN: 0022-1147. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Section cross-reference(s): 7 The influence of rice bran water-sol. hemicellulose [9034-32-6] on hydrolysis of fat by pancreatic lipase [9001-62-1] with and without bile salts was examd. in vitro. Bile salts were required for emulsification of the oil. Three of the major salts found in human bile Na cholate [81-25-4], glycocholate [475-31-0], and Na taurocholate [145-42-6] were tested for emulsifying properties with and without rice hemicellulose. The lipase activity decreased with increased addn. of hemicellulose to the bile salt-enzyme reaction mixt. The results suggest that rice hemicellulose affects lipase activity indirectly by rendering the bile salts less available for emulsification of the oil and subsequent hydrolysis by the lipase.