Reference

Conjugated ursodeoxycholic acids

Conjugated ursodeoxycholic acids. Sawada, Haruji; Watanuki, Masaaki (Yakult Honsha Co., Ltd., Japan). Eur. Pat. Appl. EP 119040 A1 19 Sep 1984, 25 pp. DESIGNATED STATES: R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: C12P033-06; C07J041-00; C07J009-00. APPLICATION: EP 84-301378 2 Mar 1984. PRIORITY: JP 83-35554 4 Mar 1983. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Ursodeoxycholic acid (I) [128-13-2] conjugated to an amino acid is produced by hydroxylation of a conjugated lithocholic acid with Mortierella ramanniana. Thus, a preculture of M. ramanniana Y2-1 was inoculated into 6 L of a pH 6 medium contg. taurolithocholic acid (II) [516-90-5] 0.5, glucose 50, polypeptone 5, yeast ext. 2, K2HPO4 2, KH2PO4 1, MgSO4.7H2O 0.5 g, CaCl2 10, FeSO4.7H2O 10, and thiamin 10 mg/L and incubated at 27° for 5 days with stirring and aeration. Tauroursodeoxycholic acid (III) [14605-22-2] was purified from the broth filtrate by chromatog. on an ion-exchanger and on silica gel. II was crystd. from EtOH-EtOAc to produce 0.49 g crystals/L broth, a 95% yield. I is produced from III by deconjugation.

Intestinal absorption of ursodeoxycholic, glycoursodeoxycholic and tauroursodeoxycholic acids in rats

Intestinal absorption of ursodeoxycholic, glycoursodeoxycholic and tauroursodeoxycholic acids in rats. Ota, Masamichi; Minami, Yasumasa; Hoshita, Takahiko (Sch. Med., Hiroshima Univ., Hiroshima 734, Japan). J. Pharmacobio-Dyn., 8(2), 114-18 (English) 1985. CODEN: JOPHDQ. ISSN: 0386-846X. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The intestinal absorption of ursodeoxycholic acid (UDC) [128-13-2], glycoursodeoxycholic acid (GUDC) [64480-66-6] and tauroursodeoxycholic acid (TUDC) [14605-22-2] was studied using an everted gut sac technique. UDC was absorbed throughout rat small intestine almost to the same extent. Absorption of both GUDC and TUDC, however, varied between jejunum and ileum. Absorption of these conjugated bile acids in the jejunal segments was less than that of UDC. While, absorption of GUDC and TUDC in the terminal ileum was more efficient than UDC. Although 2,4-dinitrophenol had no effect on the jejunal uptake, ileal uptake of these 3 bile acids was inhibited by 2,4-dinitrophenol.