Detail of "1463-10-1"

CAS Number:
1463-10-1
Name:
Ribosylthymine
Molecular Structure:
Formula:
C₁₀H₁₄N₂O₆
Molecular Weight:
258.23
Deleted CAS:
849658-09-9
Synonyms:
1-[(2R,3R,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-pyrimidine-2,4-dione;Uridine,5-methyl-;5-Methyl uridine;Thymine riboside;Methyluridine;5-Methyluridin, Ribothymidine;
EINECS:
215-973-9
Density:
1.576 g/cm³
Melting Point:
183-184 °C(lit.)
Appearance:
white powder
Hazard Symbols:
T+
Risk Codes:
26/27/28
Safety:
24/25 Details

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Reference

Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios: Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. Nguyen, Allen; Zhao, Connie; Dorris, David; Mazumder, Abhijit ( Motorola Life Sciences, Northbrook, IL 60062, USA). BMC Biotechnology [online computer file], 2, No pp. 2096-10-8 and 1463-10-1 are also occured in this study. given (English) 2002 BioMed Central Ltd. URL: http://www.biomedcentral.com/1472-6750/2/14. CODEN: BBMIE6. ISSN: 1472-6750. DOCUMENT TYPE: Journal; (online computer file) CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 9 The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful anal. tools is the inability to detect certain transcripts in the target sample because of artifacts caused by background noise or poor hybridization kinetics. The use of base-modified analogs of nucleoside triphosphates has been shown to increase complementary duplex stability in other application, and here we attempted to enhance microarray hybridization signal across a wide range of sequences and expression levels by incorporating these nucleotides into labeled cRNA targets. RNA samples contg. 2-aminoadenosine showed increases in signal intensity for a majority of the sequences. These results were similar, and additive, to those seen with an increase in the hybridization time. In contrast, 5-methyluridine and 5-methylcytidine decreased signal intensities. Hybridization specificity, as assessed by mismatch controls, was dependent on both target sequence and extent of substitution with the modified nucleotide. Concurrent incorporation of modified and unmodified ATP in a 1:1 ratio resulted in significantly greater nos. of above- threshold ratio calls across tissues, while preserving ratio integrity and reproducibility. Incorporation of 2-aminoadenosine triphosphate into cRNA targets is a promising method for increasing signal detection in microarrays. Furthermore, this approach can be optimized to minimize impact on yield of amplified material and to increase the no. of expression changes that can be detected. .

Effect of a new fungicide (PNDP) on the minor nucleotide content of Fusarium oxysporum: Effect of a new fungicide (PNDP) on the minor nucleotide content of Fusarium oxysporum. Jakucs, Erzsebet; Racz, Ilona; Lasztity, D. (Dep. Microbiol., Eotvos Lorand Univ., Budapest H-1088, Hung.). Acta Microbiol. Hung., 31(1), 27-33 (English) 1984. CODEN: AMHUEF. DOCUMENT TYPE: Journal CA Section: 5 (Agrochemical Bioregulators) Fifteen minor nucleotides were identified in 18S rRNA from F. oxysporum grown for 8 days in aerated liq. culture. These were: 6-methyladenine [443-72-1], 1-methyladenine [5142-22-3], 2-methyladenine [1445-08-5], 2,6-dimethyladenine (m62 A) [90375-79-4], 1-methylguanosine [2140-65-0], 2-methylguanosine (m2G) [2140-77-4], 2,2-dimethylguanosine [2140-67-2], 3-methylcytidine (m3C) [2140-64-9], 2'-O-methylcytidine (CM) [2140-72-9], 5-methylcytidine (m5C) [2140-61-6], 3-methyluridine [2140-69-4], 2'-O-methyluridine [2140-76-3], 5-carboxymethyluridine [20964-06-1], ribothymidine [1463-10-1] and pseudouridine (Y) [1445-07-4]. M62A, m2G, m3C, Cm, m5C, and Y were not detected in 25S rRNA. Treatment with PNDP [87810-43-3] decreased the content of nucleotides of F. oxysporum. 5-Methoxycarbonylmethyl-2-thiouridine [20299-15-4] was identified in RNA of the PNDP-treated fungus. Apparently, PNDP inhibited the synthesis of RNA-modifying enzymes of the fungus, similarly to cycloheximide.

Detail of "1463-10-1"

Ribosylthymine

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Reference