Detail of "1483-07-4"

Reference

Development of a cloning system for the gene for asparagine synthetase

Development of a cloning system for the gene for asparagine synthetase. Ray, Peter N.; Andrulis, Irene L.; Siminovitch, Louis (Dep. Genet., Hosp. Sick Child., Toronto, ON M5G 1X8, Can.). Prog. Cancer Res. Ther., 30(Gene Transfer Cancer), 59-65 (English) 1984. CODEN: PCRTDK. ISSN: 0145-3726. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 13 Two different drugs [b-aspartylhydroxamate (b-AHA) [1955-68-6] and albizziin (Alb) [1483-07-4]] and 2 selective protocols were used to select mutations involving asparagine synthetase (AS) [9023-69-2] in CHO cells. With b-AHA, single-step mutants were produced that were resistant to the drug due to structural changes in AS. Mutant selection with Alb gave resistant lines with elevated AS activities and large, homogeneously staining regions in the karyotype. These mutants led to the development of 2 methods for cloning the AS gene. One method involves rescuing the gene (by selecting for b-AHA resistance) after transfer into COS-1 cells. The other method involves constructing cDNA clones from mRNA isolated from the highly amplified Alb-resistant lines on the assumption that the 300-fold increase in enzyme activity is reflected in a comparable increase in mRNA.

In vitro and in vivo antiviral activities of L-b-(5-hydroxy-2-pyridyl)alanine (SF-1346) and L-b-(3-hydroxyureido)alanine (SF-1293B) and their related amino acid analogs

In vitro and in vivo antiviral activities of L-b-(5-hydroxy-2-pyridyl)alanine (SF-1346) and L-b-(3-hydroxyureido)alanine (SF-1293B) and their related amino acid analogs. Fukuyasu, Harumi; Kawakami, Katsuo; Sato, Yasumi; Ohya, Zenichiro; Kikuchi, Takahiro; Tsuruoka, Takashi; Inouye, Shigeharu (Cent. Res. Lab., Meiji Seika Kaisha Ltd., Yokohama 222, Japan). Meiji Seika Kenkyu Nempo, (22), 9-17 (English) 1983. CODEN: MSKNA9. ISSN: 0465-6105. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The antiviral activities of L-b-(5-hydroxy-2-pyridyl)alanine (I) [58525-82-9], L-b-(3-hydroxyureido)alanine (II) [56073-44-0], and some derivs. and analogs were examd. against 4 RNA and 2 DNA viruses in cell culture systems, in ovo, and in vivo, using mice, chicks and fish. I was active against vaccinia and influenza A viral infections and II against Newcastle disease, vaccinia, influenza A, herpes, and infectious pancreatic necrosis viral infections. L-b-Ureidoalanine [1483-07-4] showed activity against Newcastle disease and herpes viral infections.