Detail of "149-87-1"

CAS Number:
149-87-1
Name:
Proline,5-oxo-
Superlist Name:
DL-Pyroglutamic acid
Molecular Structure:
Formula:
C₅H₇NO₃
Molecular Weight:
129.11
Synonyms:
Proline, 5-oxo-, DL- (8CI);5-Oxo-DL-proline;DL-2-Pyrrolidinone-5-carboxylic acid;DL-2-Pyrrolidone-5-carboxylic acid;DL-Pyroglutamate;DL-Pyrrolidonecarboxylic acid;NSC 40887;DL-Proline,5-oxo-;
EINECS:
205-748-3
Density:
1.38 g/cm³
Melting Point:
183-185 °C(lit.)
Boiling Point:
453.1 °C at 760 mmHg
Flash Point:
227.8 °C
Solubility:
water5.67 g/100 mL (20 °C)
Appearance:
white crystalline powder
Hazard Symbols:
Xn,Xi
Risk Codes:
22-36/37/38
Safety:
26-37/39 Details

L-Proline by fermentation: L-Proline by fermentation. Nakanishi, Toshihide; Hagino, Hiroshi (Kyowa Hakko Kogyo Co., Ltd., Japan). U.S. US 4444885 A 24 Apr 1984, 4 pp. Cont. of U.S. Ser. No. 270,433, abandoned. (English). (United States of America). CODEN: USXXAM. CLASS: IC: C08G073-08. NCL: 435107000. APPLICATION: US 83-492092 6 May 1983. PRIORITY: JP 80-UT74926 5 Jun 1980; US 81-270433 4 Jun 1981. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) L-Proline [147-85-3] is produced by fermn. in the presence of L-glutamate [56-86-0] and D- [4042-36-8], L- [98-79-3], or DL-pyrrolidonecarboxylic acid [149-87-1]. Thus, a preculture of Corynebacterium acetophilum was inoculated into a pH 7.4 medium contg. Na glutamate 50, soybean meal hydrolyzate 20, KH2PO4 0.6, MgSO4.7H2O 0.6, (NH4)2SO4 23.4, FeSO4.7H2O 0.012, CaCO3 30, molasses 170 g/L (as sucrose), and thiamin HCl 100 mg/L and incubated at 28° for 4 days with shaking. The yield of proline was 39.1 g/L, compared with 25.1 g/L in a medium lacking glutamate.

Microbial production of pyroglutamate hydrolase: Microbial production of pyroglutamate hydrolase. (Noda Institute for Scientific Research, Japan). Jpn. Tokkyo Koho JP 59037073 B4 7 Sep 1984 Showa, 6 pp. (Japanese). (Japan). CODEN: JAXXAD. CLASS: IC: C12N009-86. ICI: C12N009-86, C12R001-05; C12N009-86, C12R001-13; C12N009-86, C12R001-01. APPLICATION: JP 76-156397 27 Dec 1976. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Pyroglutamate hydrolase (I) [9075-46-1] is manufd. by fermn. with Alcaligenes, Brevibacterium, or Acinetobacter strains by cultivation in the presence of L- [98-79-3] and (or) DL-pyroglutamate [149-87-1]. Thus, Alcaligenes faecalis ATCC 8750 was incubated in a pH 6.0 medium contg. KH2PO4 0.1, K2HPO4 0.1, FeSO4.7H2O 0.001, MgSO4.7H2O 0.05, and yeast ext. 0.2% in the presence of 1.5% L-pyroglutamic acid at 30° for 16 h. The cell mass was suspended in a 0.01M phosphate buffer (pH 7.0) and lysed by sonication to obtain a crude I ext. with specific activity of 0.025 units/mg proteins. Purifn. of the crude I ext. by (NH4)2SO4 fractionation, DEAE-cellulose chromatog., and Sephadex G-200 gel filtration yielded a product with specific activity of 0.4277 units/mg at 18% yield.