Detail of "14930-96-2"

Reference

Dihydrocytochalasin B

Dihydrocytochalasin B. Biological effects and binding to 3T3 cells. Atlas, Susan J.; Lin, Shin (Dep. Biophys., Johns Hopkins Univ., Baltimore, Md., USA). J. Cell Biol., 76(2), 360-70 (English) 1978. CODEN: JCLBA3. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) Dihydrocytochalasin B (I) [39156-67-7] did not inhibit sugar uptake in BALB/c 3T3 cells. Excess I did not affect inhibition of sugar uptake by cytochalasin B (II) [14930-96-2]. As in the case of II, I inhibited cytokinesis and changed the morphol. of the cells. Comparison of the effects of I, II, and cytochalasin D (III) [22144-77-0] indicated that treatment of cells with any 1 of the compds. resulted in the same series of morphol. changes; the cells underwent zeiosis and elongation at 2-4 mM II and became arborized and rounded up at 10-50 mM I. II was slightly less potent than I, whereas III was 5-8-fold more potent than II in causing a given state of morphol. change. Competitive binding studies indicated that excess I displaced essentially all of the high-affinity bound II-3H, but, at <5 ′ 10-5M. I was not as efficient as unlabeled II in the displacement reaction. In contrast, excess III displaced up to 40% of the bound II-3H. Apparently, 3 different classes of high-affinity II binding sites exist in 3T3 cells: sites related to sugar transport, sites related to cell motility and morphol., and sites with undetd. function.

Subcellular effects of cytochalasin B and dimethyl sulfoxide on Paramecium aurelia

Subcellular effects of cytochalasin B and dimethyl sulfoxide on Paramecium aurelia. Sibley, Jane T.; Paul, Matthew D.; Hanson, Earl D. (Shanklin Lab. Biol., Wesleyan Univ., Middletown, Conn., USA). J. Protozool., 24(4), 595-604 (English) 1977. CODEN: JPROAR. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) In P. aurelia cultures, DMSO [67-68-5] (2-20%) caused nuclear abnormalities, membrane degrdn., polysomal breakdown, smaller cell size, decreased survival, decreased fission rate, and inhibited leucine-3H incorporation. Cytochalasin B (I) [14930-96-2] (20-70 mg/mL)-treated cells had slower divisions and poorer survival rates than cells exposed to the equiv. DMSO concn., although the leucin-3H incorporation was greater at the lower I concns. than for DMSO alone. I caused the formation of aberrant membrane structures and ribosomal tetramers, crystals, and tubes. DMSO and I may act together to cause effects previously ascribed solely to I.