Reference

Blood histamine levels following Tavegyl administration

Blood histamine levels following Tavegyl administration. Lancucki, Jan; Nowik, Maria (Mil. Med. Acad., Warsaw, Pol.). Lek. Wojsk., 52(6), 333-6 (Polish) 1976. CODEN: LEKWAT. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Tavegyl (I) [14976-57-9] (1 mg) caused a small decrease of the histamine [51-45-6] content in whole blood, whereas a dose of 2 mg I produced greater decreases in histamine levels in serum as well as in whole blood.

Effect of drugs on human erythrocytes

Effect of drugs on human erythrocytes. II. A possible mechanism of drug-induced hemolysis. Ogiso, Taro; Kurobe, Masayuki; Masuda, Hiroyuki; Kato, Yoshio (Gifu Coll. Pharm., Gifu, Japan). Chem. Pharm. Bull., 25(5), 1078-88 (English) 1977. CODEN: CPBTAL. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) To clarify the mechanism of hemolysis, the effect of chlorpromazine-HCl (I-HCl) [69-09-0] and clemastine fumarate (II fumarate) [14976-57-9] on the structure and arrangement of the membrane proteins and phospholipids was studied using CD and some hydrophobic probes, in addn. to studies on the alterations in the components of the cells and using "pink ghosts" contg. .alpha.-amylase. Initial expts. demonstrated that human erythrocytes were hemolyzed within several minutes at a higher drug concn. (1 .times. 10-3 or 8 .times. 10-4M), while a somewhat lower concn. (6 or 5 .times. 10-4M) there was a lag phase for about 30 min and then resulted in severe hemolysis. Dramatic hemolysis occurred at a I concn. which approx. corresponds with the crit. micellar concn. The content of sulfhydryl groups in the cells and calcium in the membrane was not affected by the drug treatment. At above 2 .times. 10-4M of both drugs, components I and II (spectrin) were released from the membrane. These drugs, however, had little or no effect on the CD spectra of the ghosts, suggesting that the drug-induced hemolysis is not due to the denaturation of the protein. 1-Anilinonaphthalene 8-sulfonate (ANS)-ghost fluorescence and 2,4,6-trinitrobenzenesulfonate (TNBS) binding to aminophospholipids were extremely enhanced by the drug exposure. The enhancement of the ANS-ghost fluorescence and TNBS binding with drugs was almost paralleled with that of the hemolytic effect. These drugs thus disturb the arrangement of phospholipids and the hydrophobic interactions between lipids and proteins, and alter the membrane permeability, thereby appear to induce hemolysis.