Detail of "15673-79-7"

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Practical synthesis of 5-phospho-D-ribosyl a-1-pyrophosphate (PRPP): enzymatic routes from ribose 5-phosphate or ribose

Practical synthesis of 5-phospho-D-ribosyl a-1-pyrophosphate (PRPP): enzymatic routes from ribose 5-phosphate or ribose. Gross, Akiva; Abril, Obsidiana; Lewis, Jerome M.; Geresh, Shimona; Whitesides, George M. (Dep. Chem., Harvard Univ., Cambridge, MA 02138, USA). J. Am. Chem. Soc.Several reagents with their cas registry numbers 15673-79-7 and 25014-41-9 are used here., 105(25), 7428-35 (English) 1983. CODEN: JACSAT. ISSN: 0002-7863. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 7, 33 Enzymic syntheses of PRPP on a 75-mmol scale are described. The reactions used polyacrylonitrile (PAN)-immobilized PRPP synthetase as catalyst with in situ ATP-cofactor regeneration. In one procedure, pure ribose 5-phosphate (r-5-P) was used as a starting material; in a 2nd method, r-5-P was synthesized by ribokinase-catalyzed phosphorylation of D-ribose and used in situ. The potential for use of PRPP as a starting material for the prepn. of nucleotides was demonstrated by the enzymic synthesis of UMP. Several methods also are described for the prepn. of r-5-P: acid-catalyzed hydrolysis of AMP, acid-catalyzed hydrolysis of a crude mononucleotide mixt. obtained by digestion of RNA, chem. synthesis from D-ribose, and ribokinase-catalyzed synthesis from D-ribose. Procedures are described for the isolation of PRPP synthetase (from Salmonella typhimurium) and ribokinase (from Lactobacillus plantarum) and for the immobilization of these enzymes in PAN. .