Detail of "16002-19-0"

Reference

Dissociation of malondialdehyde mutagenicity in Salmonella typhimurium from its ability to induce interstrand DNA cross-links

Dissociation of malondialdehyde mutagenicity in Salmonella typhimurium from its ability to induce interstrand DNA cross-links. Basu, Ashis K.; Marnett, Lawrence J.; Romano, Louis J. (Dep. Chem., Wayne State Univ., Detroit, MI 48202, USA). Mutat. Res., 129(1), 39-46 (English) 1984. CODEN: MUREAV. ISSN: 0027-5107. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Malondialdehyde (MDA) [542-78-9], an in vivo metabolite of lipid peroxidn. and prostaglandin biosynthesis, was mutagenic in S. typhimurium. To explore the possibility that MDA-induced interstrand cross-links were a premutagenic lesion, the ability of MDA to form interstrand cross-links when reacted with linear plasmid DNA was studied. At physiol. temp. and pH, MDA did not form DNA cross-links as detd. by DNA denaturation followed by agarose gel electrophoresis. DNA cross-links were formed, however, when incubations with MDA were carried out at either pH 4.2 or temps. exceeding 60°. a-Methylmalondialdehyde [16002-19-0] cross-linked with DNA more efficiently than MDA, but was not mutagenic in any tester strain of Salmonella. MDA polymers formed by acid incubation of MDA also were capable of inducing cross-links. However, an inverse relation was obsd. between mutagenicity and the extent of polymn. The pattern of mutagenic response for MDA in different strains of Salmonella was compared with mitomycin C [50-07-7], an established mutagenic cross-linking agent. Error-prone repair and a UvrB+ phenotype, which were needed for the induction of mutations by mitomycin C, were not required for MDA mutagenesis. These findings, taken together, dissoc. the mutagenicity of MDA from its ability to form interstrand cross-links with DNA.