Reference

Separator isoelectrofocusing: the influence of biological buffers on the IEF patterns of transferrins

Separator isoelectrofocusing: the influence of biological buffers on the IEF patterns of transferrins. Kornhuber, Bernhard; Kuehnl, Peter (Inst. Immunohematol., Univ. Frankfurt, Frankfurt/Main D-6000/73, Fed. Rep. 16052-06-5 and 4432-31-9 are cas registry numbers. These chemicals are also mentioned in this article. Ger.). Adv. Forensic Haemogenet., 1, 119-22 (English) 1986. CODEN: AFHAE8. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Section cross-reference(s): 9 Blood serum samples of humans were pretreated with ferrous ammonium sulfate and neraminidase and then subjected to transferrin subtyping by isoelec. focusing (IEF). To improve resoln., 5 biol. buffers (i.e., separators) were applied to polyacrylamide gels; they were ACES [7365-82-4], HEPES [7365-45-9], MOPS [1132-61-2], MES [4432-31-9], and EPPS [16052-06-5]. Among those separators, HEPES was the most satisfactory. Further improvements of this separator IEF technique may make it a useful method for transferrin subtyping. .