Reference

On the amounts of urethane formed in diethyl pyrocarbonate treated beverages

On the amounts of urethane formed in diethyl pyrocarbonate treated beverages. Solymosy, F.; Antoni, F.; Fedorcsak, I. (Inst. Biochem. 1, Semmelweis Univ. Med. Sch., Budapest, Hung.). J. Agric. Food Chem., 26(2), 500-3 (English) 1978. CODEN: JAFCAU. DOCUMENT TYPE: Journal CA Section: 16 (Fermentations) Exptl. evidence is presented to show that by reaction kinetic calcns. the upper limit of the amt. of urethane [51-79-6] formed in NH3 [7664-41-7]-contg. beverages treated with diethyl pyrocarbonate [1609-47-8] under defined conditions can be predicted. This offers a simple and unequivocal way of deciding whether or not a wine sample of a given pH and NH3 content can be treated with di-Et pyrocarbonate so as not to exceed the permissible level of urethane, 10 mg/L.

Histidine modification with diethylpyrocarbonate induces a decrease in the binding of an antagonist, PK 11195, but not of an agonist, RO5-4864, of the peripheral benzodiazepine receptors

Histidine modification with diethylpyrocarbonate induces a decrease in the binding of an antagonist, PK 11195, but not of an agonist, RO5-4864, of the peripheral benzodiazepine receptors. Benavides, J.; Begassat, F.; Phan, T.; Tur, C.; Uzan, A.; Renault, C.; Dubroeucq, M. C.; Gueremy, C.; Le Fur, G. (PHARMUKA Lab., Gennevilliers 92231, Fr.). Life Sci., 35(12), 1249-56 (English) 1984. CODEN: LIFSAK. ISSN: 0024-3205. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) 3H-labeled PK-11195 (I) [85532-75-8] binding to peripheral type benzodiazepine binding sites n kidney membranes is inhibited by the histidine [71-00-1] blocking agent diethylpyrocarbonate [1609-47-8]. This reagent irreversibly decreases the Bmax for oH-labeled PK-11195 [85532-75-8] without affecting the affinity. By contrast, binding of [3H]RO5-4864 is not affected by diethylpyrocarbonate treatment. However RO5-4864 can protect in a concn. dependent manner the [3H]PK-11195 binding site from diethylpyrocarbonate whereas clonazepam and RO15-1788 are not active. Apparently, PK-11195 is an antagonist and RO5-486 an agonist at the "peripheral type" benzodiazepine receptors.