Reference

Dioxetane chemiluminescence detection in liquid chromatography

Dioxetane chemiluminescence detection in liquid chromatography. Niederlander, H. A. G.; Van Assema, W.; Engelaer, F. W.; Gooijer, C.; Velthorst, N. H. (Dep. Gen. Anal. Chem., Free Univ., Amsterdam 1081 NV, Neth.). Anal. Chim. Acta, 255(2), 395-401 (English) 1991. CODEN: ACACAM. ISSN: 0003-2670. DOCUMENT TYPE: Journal CA Section: 80 (Organic Analytical Chemistry) A study of the potential of dioxetane chemiluminescence (CL) detection in reversed-phase column liq. chromatog. is presented. 3,4-Diethoxy-1,2-dioxetane (DEDO) is generated online in a post-column photochem. reactor from 1,2-diethoxyethylene (DEE) and singlet oxygen; the latter is produced photochem. by the eluting compds. via oxygen quenching. CL is obtained by thermal decompn. of DEDO in the detector cell (kept at 70. 53469-21-9 and 16484-86-9 are cas registry numbers of chemicals which are used as reagents here.0°C) in the presence of 9,10-dibromoanthracene (DBA). In the exptl. set-up two post-column reagent addn. lines are applied, providing DEE in acetonitrile before and DBA in THF after the photochem. reactor. The photochem. reactor is a knitted PTFE tube, irradiated with a medium-pressure mercury lamp in combination with a filter cuvette to prevent autoxidn. of DEE. The exptl. conditions were optimized. Reversed-phase chromatograms applying exclusively acetonitrile-water (90 + 10, vol./vol.) are presented and CL detection is compared with UV absorption detection. For a no. of analytes the concn. detection limits in both techniques are similar, i.e., in the 10-8-10-7M range. Other compds., although providing strong absorption signals, hardly provide any CL, thus indicating the selectivity of the new technique. .