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Determination of a new carbapenem antibiotic, DA-1131, in rat plasma, urine, and bile by column-switching high-performance liquid chromatography

Determination of a new carbapenem antibiotic, DA-1131, in rat plasma, urine, and bile by column-switching high-performance liquid chromatography. Lee, Eung D.; Lee, Sang D.; Kim, Won B.; Yang, Junnick; Kim, So H.; Lee, Myung G. (Res. Lab., Dong-A Pharmaceutical Company, Ltd., Kyunggi-Do 449-900, S. Korea). Research Communications in Molecular Pathology and Pharmacology, 94(2), 171-180 (English) 1996 PJD Publications. CODEN: RCMPE6. ISSN: 1078-0297. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A column-switching high-performance liq. chromatog. method has been developed for the detn. of a new carbapenem antibiotic, DA-1131, in rat plasma, urine, and bile. Each biol. sample was dild. with an equal vol. of a stabilizer, 3% KH2PO4 (contg. 2.0% sodium citrate, adjusted to pH 6.0 with 10 M NaOH). After centrifugation for 1 min at 3000 g, an aliquot of the supernatant was injected directly onto the HPLC column. Deionized water was run for 3 min at a flow rate of 1.5 mL/min to retain DA-1131 in an extn. column, while proteins and endogenous interferences were eluted to the waste. The analyte was then back-flushed onto an anal. column, C18 reversed-phase column. The mobile phases for anal. column, 10 mM 3-[N-morpholino]propane sulfonic acid (pH 4.5)-acetonitrile (, vol./vol.) for plasma samples, and 5 mM KH2PO4 (adjusted to pH 8.0 with 10 M NaOH)-acetonitrile (, vol./vol.) for urine and bile samples, were run at a flow rate of 1.0 mL/min. The column effluent was monitored by UV detection at 300 nm. 169285-98-7 which is the cas registry number of one of substances is just one of reagents here. The retention time for DA-1131 was 8 min in plasma samples and 13 min for urine and bile samples. The detection limits for DA-1131 in rat plasma, urine, and bile were 0.05, 1.0, and 1.0 mg/mL, resp. The intraday and interday coeffs. of variation of the assay were generally low (below 8.40%) for rat plasma, urine, and bile samples. No interference from endogenous substances was obsd. .

Determination of a new carbapenem derivative, DA-1131, in plasma and urine by high-performance liquid chromatography

Determination of a new carbapenem derivative, DA-1131, in plasma and urine by high-performance liquid chromatography. Kim, So Hee; Kwon, Jong Won; Yang, Junnick; Lee, Myung Gull ( College of Pharmacy, Seoul National University, San 56-1, Shinlim-Dong, Kwanak-Gu, Seoul, S. Korea). Journal of Chromatography, B: Biomedical Applications, 688(1), 95-99 (English) 1997 Elsevier.In this study，169285-98-7 is also used. CODEN: JCBBEP. ISSN: 0378-4347. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) A high-performance liq. chromatog. method was developed for the detn. of a new carbapenem, DA-1131 (I), in human plasma and urine and in rat blood and tissue homogenates. The method involved deproteinization of the biol. samples with 1 vol. each of 0.04 M Ba(OH)2 and ZnSO4 aq. soln. A 50-ml aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was 0.015 M KH2PO4-acetonitrile (9:1, vol./vol.) with a pH of 5.0. The flow-rate was 0.8 mL/min. The column effluent was monitored by a UV detector at 300 nm. The retention time of I was 8.0 min. The detection limits of I in human plasma and urine were 0.1 and 0.5 mg/mL, resp. The coeffs. of variation of the assay were generally low (below 8.39) for human plasma and urine, and rat blood and tissue homogenates. No interferences from endogenous substances were obsd. .