Detail of "17817-20-8"

Reference

Nuclear medicine

Nuclear medicine. Lin, Xiangtong (Department of Nuclear medicine, Huashan Hospital, Fudan University, Shanghai 200040, Peop. Rep. China). Zhonghua Yixue Zazhi (Beijing, China), 82(24), 1719-1721 (Chinese) 2002 Zhonghua Yixue Zazhishe. CODEN: CHHTAT. ISSN: 0376-2491. DOCUMENT TYPE: Journal; General Review CA Section: 8 (Radiation Biochemistry) A review with 12 refs. on nuclear medicine with subdivision headings: (1) position emission tomog.; (2) single photon emission computed tomog.; (3) research of radial imaging agent.Except for chemicals metioned above, 17817-20-8 is also used. .

Selection of rare event cells expressing b-galactosidase

Selection of rare event cells expressing b-galactosidase. Jiwa, Asmina H.; Wilson, James M. (Meridian Instrum., Inc., Okemos, MI 48864, USA). Methods (San Diego), 2(3), 272-80 (English) 1991. CODEN: MTHDE9. ISSN: 1046-2023. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) The ACAS Interactive Laser Cytometer was used to screen and select mammalian cells in culture infected with a retrovirus construct contg. the Escherichia coli lacZ and neomycin resistance genes. The product of expression of the lacZ gene, b-galactosidase, can be quantitated using the fluorescent substrate fluorescein di-b-D-galactopyranoside (FDG). NIH 3T3 cells infected with the retrovirus construct were selected for neomycin resistance and individual colonies were cloned. These cells served as a pos. control, and uninfected parent NIH 3T3 cells were used as the neg. control. Autofluorescence and background fluorescence using FDG were examd.Several substances with their cas registry numbers 9031-11-2 and 17817-20-8 may be metioned in this study. on uninfected NIH 3T3 cells and found to be negligible. The fluorescence assay was performed on individual adherent cells in culture. Results indicate that cells expressing the recombinant retrovirus product, b-galactosidase, can be viably stained in culture, analyzed, sorted, and cloned without the cells being trypsinized or removed from their natural growth surface. The assay is sensitive enough to detect lacZ+ and lacZ- cells and also capable of discriminating between high and low expressors of b-galactosidase. This technol. could be a powerful tool in gene therapy and gene regulation studies. By using the b-galactosidase gene as the reporter mol. in the constructs contg. the gene of the reporter mol. in the constructs contg. the gene of interest, selection of infected/transfected cells, infection/transfection efficiency, promoter strength, and specificity can be measured with minimal manipulation soon after the infection/transfection process. This technique can be further extended for sorting mutants cells, hybridomas, and rare event occurrences. .