Reference

High-pressure liquid chromatographic and other assays for biphenyl hydroxylation compared

High-pressure liquid chromatographic and other assays for biphenyl hydroxylation compared. Burke, M. D.; Benford, D. J.; Bridges, J. W.; Parke, D. V. (Dep. Biochem., Univ. Surrey, Guildford, Engl.). Biochem. Soc. Trans., 5(5), 1370-2 (English) 1977. CODEN: BCSTB5. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Two chromatog. assays for 2- [90-43-7], 3- [580-51-8], and 4-hydroxybiphenyl [92-69-3] without interference from metabolites of carcinogens such as benzo[a]pyrene (I) [50-32-8] were developed. The min. quantities measured by the high pressure liq. chromatog. were 0.02 nmol 2-, 3-, and 4-hydroxybiphenyl, 0.01 nmol 2,2'- [1806-29-7], and 0.05 nmol 4,4'-dihydroxybiphenyl [92-88-6]. I metabolites were probably eluted in the column void vol. The min quantities of hydroxylated biphenyls measured by the gas-liq.-chromatog. method were 0.75 nmol. The modified microsomal I metabolites were probably destroyed during the assay.

Assay of underivatized biphenyl metabolites by high-pressure liquid chromatography

Assay of underivatized biphenyl metabolites by high-pressure liquid chromatography. Burke, M. Danny; Prough, Russell A. (Dep. Forensic Med., Karolinska Inst., Stockholm, Swed.). Anal. Biochem., 83(2), 466-73 (English) 1977. CODEN: ANBCA2. ISSN: 0003-2697. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Biphenyl [92-52-4] and its hydroxylated derivs. were sepd. and assayed by high-pressure liq. chromatog., using a mBondapack-NH2 column eluted with isooctane-acetonitrile-isoamyl alc. (:4), into which was incorporated 10% (v/v) ethanol toward the end of the sepn. The retention times, in min, were biphenyl, 1.0; 2-hydroxybiphenyl [90-43-7], 4.0; 3-hydroxybiphenyl [580-51-8], 6.3; 4-hydroxybiphenyl [92-69-3], 7.6; 2,2'-dihydroxybiphenyl [1806-29-7], 12.9; and 4,4'-dihydroxybiphenyl [92-88-6], 15.0. Using this method to assay biphenyl metabolites formed in vitro and in vivo, it was found that control rat or rabbit liver homogenate or microsomes formed as the only major metabolite, 4-hydroxybiphenyl. This was also the only appreciable biphenyl metabolite in acid-hydrolyzed rat urine, with trace amts. of 2-hydroxy- and 4,4'-dihydroxybiphenyl. Liver microsomes of 3-methylcholanthrene [56-49-5]-treated rats, however, formed the 2-, 3-, and 4-hydroxybiphenyls.