Detail of "181952-24-9"

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Preparation of acidic peptides for use in isolation and anal

Preparation of acidic peptides for use in isolation and anal. of living body components. Imajo, Nobuko; Yamagata, Yukari; Katoh, Hideo; Satomura, Shinji; Nakamura, Kenji (Wako Pure Chemical Industries, Ltd., Japan). Eur. Pat. Appl. EP 755941 A2 29 Jan 1997, 42 pp. DESIGNATED STATES: R: DE, ES, FR, GB, IT. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: C07K001-00. ICS: C07K005-02; C07K005-06; C07K005-08; C07K005-10; C07K007-02; C07K007-06. APPLICATION: EP 1996-305255 17 Jul 1996. PRIORITY: JP 1995-203886 18 Jul 1995; JP 1996-84770 13 Mar 1996. DOCUMENT TYPE: Patent CA Section: 34 (Amino Acids, Peptides, and Proteins) Section cross-reference(s): 9, 15 A living body component in a sample derived from a living body can be rapidly and accurately measured by reacting the sample with a reagent comprising a combined product of an affinity substance and a peptide having at least three acid residues derived from a strong acid, sepg. the resulting complex by a method applying neg. change such as using an anion-exchanger, and detg. the amt. of the analyte to be measured, on the basis of the amt. of the complex or free combined product.There are some commonly used reagents with their cas registry numbers 35661-39-3 and 181952-24-9 in this article. Thus, sulfotyrosine peptide H-Ala-[Tyr(SO3H)]8-b-Ala-OH (I) was prepd. by std. solid-phase methods on a Azko resin using 9-fluorenylmethoxycarbonyl (Fmoc) Na protection. I was coupled with sulfosuccinimidyl 4-(p-maleimidophenyl)butyrate and then conjugated with anti-a-fetoprotein (anti-AFP) monoclonal antibody A4-4. The HPLC Rf values of the AFP-sulfopeptide-antibody conjugate and the sulfopeptide-antibody conjugate are nearly the same, demonstrating that the neg. charge of AFP does not influence Rf values of antigen-antibody reaction products. .