Reference

The decrease of hyaluronate synthesis by anti-inflammatory steroids in vitro

The decrease of hyaluronate synthesis by anti-inflammatory steroids in vitro. Saarni, Heikki; Hopsu-Havu, V. K. (Dep. Med. Chem., Univ. Turku, Turku, Finland). Br. J. Dermatol., 98(4), 445-9 (English) 1978. CODEN: BJDEAZ. ISSN: 0007-0963. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) The effect of the antiinflammatory steroids, prednisolone (I) [50-24-8], hydrocortisone (II) [50-23-7], II Na succinate [125-04-2], II 17-butyrate [13609-67-1], dexamethasone [50-02-2], dexamethasone Na phosphate [1869-92-7], betamethasone [378-44-9], betamethasone phosphate [360-63-4], and betamethasone 17-valerate [2152-44-5], on the synthesis of hyaluronic acid [9004-61-9] and sulfated glycosaminoglycans in human skin fibroblast culture was studied. The concns. of steroids varied between 1 ′ 10-10-1 ′ 10-6M. All tested steroids decreased the synthesis of hyaluronic acid to the same final level which was ~40-50% of the controls, but the concns. required varied between different steroids. The relative inhibitory potencies of the steroids were calcd. based on concns. needed to decrease the synthesis of hyaluronate. When the inhibitory potency of II was calcd. as 1, the values of the other steroids were: I 5, II 17- butyrate 20, betamethasone alc. 30, dexamethasone alc. 38, betamethasone 17-valerate 350-400, dexamethasone monosodium phosphate and betamethasone disodium phosphate >400. II Na succinate was as potent an inhibitor of hyaluronate synthesis as II. None of the tested steroids affected the synthesis of sulfated glycosaminoglycans at these concns. The changes obsd. in the glycosaminoglycans in the medium were in accordance with the changes in the cell layer. The possible significance of hyaluronate synthesis inhibition by antiinflammatory steroids is discussed.

The effect of certain anti-inflammatory steroids on collagen synthesis in vitro

The effect of certain anti-inflammatory steroids on collagen synthesis in vitro. Saarni, Heikki (Dep. Med. Chem., Univ Turku, Turku, Finland). Biochem. Pharmacol., 26(21), 1961-6 (English) 1977. CODEN: BCPCA6. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Several antiinflammatory steroids and their derivs., but not dexamethasone (I) [50-02-2] or I monosodium phosphate [1869-92-7], at concns. of 10-3-10-7M, decreased collagen formation in chick embryo tendon cells. The amt. of labeled collagen decreased more in the medium than in the cells, but no steroid caused an accumulation of labeled collagen in the cells. Hydrocortisone (II) [50-23-7] was as inhibitory as hydrocortisone-17-butyrate [13609-67-1], but both were more effective than II Na succinate [125-04-2] or II monosodium phosphate [38048-30-5]. Betamethasone-17-valerate [2152-44-5] was the most potent inhibitor tested, but betamethasone (III) [378-44-9] and III disodium phosphate [151-73-5] were ineffective. Prednisolone [50-24-8] was as effective against collagen as II. The decrease in collagen followed the decrease in total labeled protein. Inhibition of collagen formation by antiinflammatory steroids may be a consequence of a general protein formation inhibition.