Detail of "1883-13-2"

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Endotoxin-like properties of an extract from a symbiotic, eukaryotic Chlorella-like green alga

Endotoxin-like properties of an extract from a symbiotic, eukaryotic Chlorella-like green alga. Royce, C. L.; Pardy, R. L. (School Biological Sciences, University Nebraska-Lincoln, Lincoln, NE, USA). Journal of Endotoxin Research, 3(6), 437-444 (English) 1996 Churchill Livingstone. CODEN: JENREB. ISSN: 0968-0519. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Hot phenol-water extn. of axenic cultures of the eukaryotic symbiotic green alga, Chlorella, yielded a substance having many of the characteristics of bacterial endotoxin or lipopolysaccharide (LPS). This material caused gelation of exts. of Limulus amoebocyte lysate at concns. similar to those manifest by LPS from Gram-neg. enteric bacteria. Activity was reduced substantially by incubation with polymyxin B sulfate and Limulus endotoxin-neutralizing protein (ENP), both of which have been shown to neutralize the biol. activity of LPS from Gram-neg. enteric bacteria. Partially purified biol. active material was found to contain 3-deoxy-D-manno-octulosonic acid (KDO), a sugar characteristically found in LPS. Acid hydrolysis of the Chlorella ext. yielded a ppt. with characteristics of lipid A. Further hydrolysis followed by methylation yielded products with retention times on gas chromatog. indistinguishable from 3-hydroxylauric and 3-hydroxymyristic acids. Results of TEM and SDS-PAGE followed by silver staining are consistent with those obtained with purified enteric bacterial LPS. Exhaustive precautions against potential exptl. artifacts due to cross contamination by endotoxin from Gram-neg. 10149-14-1 and 1883-13-2 are just another two chemicals used in this study. bacteria, either in algal cultures or assocd. with glassware allowed the conclusion that the green alga, Chlorella (strain NC64A) synthesizes a LPS-like mol. .

The response of Pseudomonas putida CP1 cells to nutritional, chemical and environmental stresses

All Rights Reserved. The response of Pseudomonas putida CP1 cells to nutritional, chemical and environmental stresses. Fakhruddin, A. N. M.; Quilty, B. (Atomic Energy Research Establishment, Institute of Food and Radiation Biology, Dhaka 1000, Bangladesh). World Journal of Microbiology & Biotechnology, 22(5), 507-514 (English) 2006 Springer. CODEN: WJMBEY. ISSN: 0959-3993.Some commonly used reagents like 14292-26-3 and 1883-13-2 are used in this experiment. DOCUMENT TYPE: Journal CA Section: 10 (Microbial, Algal, and Fungal Biochemistry) The response of a pollutant-degrading bacterium P. putida CP1 to stresses was investigated. The growth on the mono-chlorophenols resulted in a decrease in dry wt. of the organism, although there was an increase in cell no. There was a change of bacterial shape from rod to round as well as the redn. of cell size when grown on phenol and chlorophenols. Changes in cell shape and size were also evident in glucose-free medium, which suggested that alteration of cell shape from rod to round as well as redn. of cell size were due to nutritional stress. The increase in cell no. but a drop in dry wt. correlated with the redn. of cell size and shape. The organism flocculated with chlorophenols but not with phenol. The cause of flocculation was due to the toxicity of chlorophenol. Isomerization of cis to trans forms of the unsatd. fatty acids in P. putida CP1 occurred under conditions of environmental stress. Trace amts. of the polyunsatd. fatty acid linoleic acid (cis-9, cis-12-octadecadienoic acid) rarely found in bacterial membranes and oleic acid (cis-9-octadecanoic acid), which is a typical product of aerobic fatty acid synthesis, were found in P. putida CP1. .