Reference

Immobilizing biological materials in condensed alkylenimine polymers

Immobilizing biological materials in condensed alkylenimine polymers. Swann, W. E. (Genex Corp., USA). Belg. BE 896509 A1 16 Aug 1983, 23 pp. (French). (Belgium). CODEN: BEXXAL. ICI: C12; C12. APPLICATION: BE 21-589 19 Apr 1983. PRIORITY: US 82-370242 20 Apr 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Biol. compds. are immobilized by entrapment in alkylenimine polymers condensed with dicarboxylic acids. Thus, 4 g polyethylenimine [9002-98-6] in a 30% aq. soln. was mixed with 2 g succinic acid [110-15-6]. The soln. remained liq.; 1 mL of an aspartase [9027-30-9] was added and polymn. was induced by addn. of 1-ethyl-3,3-dimethylaminopropylcarbodiimide [1892-57-5]. The gel that formed was ground to small pieces. The prepn. was used to convert 50 mL of a 1M soln. of NH4 fumarate to 1.509 mmol. aspartic acid [56-84-8] in 1 h.

Protein composition

Protein composition. Poole, Stephen; West, Stuart Ian; Walters, Clifford Lesley (British Food Mfg. Industries Research Assoc., UK). Brit. UK Pat. Appl. GB 2134117 A1 8 Aug 1984, 15 pp. (English). (United Kingdom). CODEN: BAXXDU. CLASS: IC: C07G007-00. APPLICATION: GB 84-1387 19 Jan 1984. PRIORITY: GB 83-1764 21 Jan 1983; GB 83-1765 21 Jan 1983; GB 83-9883 12 Apr 1983. DOCUMENT TYPE: Patent CA Section: 17 (Food and Feed Chemistry) A mixt. of acidic and basic proteins has better foaming, gelling, and emulsifying properties than does acidic protein alone. Acidic proteins can be chem. modified to form basic proteins. Thus, a 2% aq. soln. of acidic whey protein isolate was adjusted to pH 6 with HCl, and glycine Me ester [616-34-2] was added at 5% followed by 1-ethyl-3-dimethylaminopropyl carbodiimide [1892-57-5] at 0.6%. The soln. was held at 26° for 48 h. This procedure modified 25% of the CO2H groups of the protein and raised the pI to >9.5. The protein could be combined with an acidic protein to improve its functional properties.