Detail of "1919-91-1"

Reference

Cytofluorometric quantification of the activity and reaction kinetics of acid phosphatase

Cytofluorometric quantification of the activity and reaction kinetics of acid phosphatase. Prenna, G.; Bottiroli, G.; Mazzini, G. (Ist. Anat. Compar., Univ. Pavia, Pavia, Italy). Histochem. J., 9(1), 15-30 (English) 1977.Several substances with their cas registry numbers 1919-91-1 and 9001-77-8 may be metioned in this study. CODEN: HISJAE. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) Fluorogenic substrates derived from naphthol AS were used for the microscopic demonstration and cytofluorometric quantification of the activity and reaction kinetics of acid phosphatase in single living cells. A special study was made of acid naphthol AS-BI phosphatase. However, the method can be extended to other hydrolytic enzymes. The method is sensitive and accurate because (1) quantification of very low enzyme activity is possible, (2) the reaction kinetics can be evaluated with a good degree of precision inasmuch as the initial reaction velocity is derived over short times, (3) there is an absence of distributional error, and (4) the errors due to extracellular diffusion of the hydrolyzed substrate, to photodecompn., and to autofluorescence can be contained within very narrow limits. The procedures for detg. enzyme activity and reaction kinetics, and the instrumental characteristics and devices required for carrying out these measurements, are described. Some possible applications are indicated. .