Reference

Analysis of ginseng sapogenin in ginseng preparations

Analysis of ginseng sapogenin in ginseng preparations. Meng, Zhengmu; Sun, Yiping (Div. Phytochem., Nanjing Coll. Pharm., Nanjing, Peop. Rep. China). Nanjing Yaoxueyuan Xuebao, (2), 7-12 (Chinese) 1983. CODEN: NYXUDF. ISSN: 0254-5055. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Ginseng root prepns. were analyzed for their contents of panaxatriol [32791-84-7], panaxadiol [19666-76-3] and oleanolic acid [508-02-1] by the dual-wavelengths TLC scanning method. Total sapogenins from the prepns. were qual. detd. by 2-dimensional TLC. Of 6 prepns. tested, total sapogenin contents ranged 27.27-293.48 mg/100 mL or 100 tablets, oleanolic acid contents ranged 9.11-105.43 mg/100 mL or 100 tablets, panaxadiol contents ranged 11.38-176.36 mg/100 mL or 100 tablets and panaxatriol contents ranged 4.48-69.36 mg/100 mL or 100 tablets. TLC patterns of constituents of 9 prepns. are presented.Except for chemicals metioned above, 32791-84-7 and 508-02-1 are also used. .

Testing of Panax ginseng C

Testing of Panax ginseng C. A. Meyer. Pleinard, J. F.; Delaveau, P.; Guernet, M. (Lab. Chim. Anal., Fac. Pharm., Chatenay-Malabry, Fr.). Ann. Pharm. Fr., 35(11-12), 465-73 (French) 1977. CODEN: APFRAD. ISSN: 0003-4509. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Section cross-reference(s): 11 Methods are described for studying ginseng roots and exts.; specifically, saponosides and sapogenins. For extg. saponosides, the technique of Shibata (1962) gave the best results. Using 2-dimensional TLC on silica gel and BuOH-AcOH-H2O (:10) or CHCl3-MeOH-H2O (:10) solvent, good resoln. was possible. Of the revealing agents used, H2SO4 gave the best coloration but diffuse spots and SbCl3 produced very intense spots. The sapogenin hydrolysis products panaxadiol (I) [19666-76-3] and panaxatriol (II) [32791-84-7] were studied with TLC and GLC. Using the above extn. procedure, the ext. was acid hydrolyzed and I and II were pptd. by the addn. of water. The compds. were extd. into CHCl3 and spotted on silica gel plates. After chromatographing with benzene-EtOAc (1:1) the spots were developed with SbCl3 in CHCl3 and heating at 120°. For GLC anal. of I and ginseng powder (1.5 g) was extd. with MeOH. After several extn. steps to purify the sample, I and II were hydrolyzed and silylated. Samples were chromatographed on a 3 m column with a stationary phase of gas chromosorb Q 80-100 mesh and 2.5% OV-101. The column temp. was 230-300° with an increase of 2°/min. N was the carrier gas and a flame ionization detector was used. Ginseng root I and II levels varied (0.07-0.16 and 0.06-0.15%, resp.) with the type of root studied, the small roots having the highest of levels.