Reference

Effects of anticoagulants on the hematocrit, osmolarity and pH of avian blood

Effects of anticoagulants on the hematocrit, osmolarity and pH of avian blood. Fourie, F. le R. (Dep. Zool., Rand Afrikaans Univ., Johannesburg, S. Afr.). Poult. Sci., 56(6), 1842-6 (English) 1977. CODEN: POSCAL. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) In pigeons (Columba livia), Na heparin [9041-08-1] produced minimal variations in blood pH, hematocrit, osmolarity, or plasma protein concns. Dipotassium EDTA [2001-94-7] affected all parameters to some extent with the exception of hematocrit which remained stable when EDTA was utilized in low concns. Disodium oxalate [62-76-0] and trisodium citrate [68-04-2] caused marked variations in the plasma protein concn., osmolarity and pH of blood samples. The effect of oxalate on pH was insignificant when utilized in low concns. but did tend to elevate the pH of blood samples when administered in higher concns. Thus, care should be exercised in the selection of anticoagulants for use in the various aspects of avian hematol.

The effect of anticoagulant on platelet associated IgG

The effect of anticoagulant on platelet associated IgG. Lucas, G. F.; Holburn, A. M. (Blood Group Ref. Lab., Radcliffe Infirm.Chemical with cas number 2001-94-7 also plays role., Oxford, UK). Br. J. Haematol., 65(1), 111-15 (English) 1987. CODEN: BJHEAL. ISSN: 0007-1048. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Blood anticoagulated with solid K2EDTA had platelet assocd. IgG (PAIgG) levels 5-fold greater than citrated blood when the platelets were harvested by differential centrifugation but identical PAIgG levels when the platelets were harvested by Percoll d. gradient. Furthermore, blood anticoagulated with increasing amts. of solid K2EDTA demonstrated a proportionate increase in both PAIgG and hemolysis whereas the same blood sample anticoagulated with aq. K2EDTA exhibited neither of these effects. Coulter Counter anal. of platelet poor plasma (PPP) prepd. from blood anticoagulated with solid K2EDTA revealed particles with a mean vol. greater than normal RBC; such particles were not found in the PPP prepd. from citrated blood. While some of these particles could be sedimented through plasma, those remaining were sufficiently buoyant to resist sedimentation unless the plasma was dild. with buffer. Studies with FITC conjugated antisera revealed that the particles could be classified into 2 types. Type I particles reacted strongly with a monoclonal anti-RBC antibody, weakly with anti-IgG antibody and had the appearance of stroma. Type II particles lacked any apparent biol. structure, reacted strongly with anti-IgA antibody but not with monoclonal anti-RBC antibody. Thus, the buoyant IgG-rich particles found in blood anticoagulated with solid K2EDTA appear to contribute to the high PAIgG levels assocd. with this anticoagulant. Consequently, solid K2EDTA should not be used to anticoagulate blood destined for PAIgG measurement. .