Detail of "2001-96-9"

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Induction of emphysematous lesions in rat lung by b-D-xyloside, an inhibitor of proteoglycan synthesis

Induction of emphysematous lesions in rat lung by b-D-xyloside, an inhibitor of proteoglycan synthesis. van Kuppevelt, Toin H.; van de Lest, Chris H.; Versteeg, Elly M. M.; Dukhuijzen, P. N. Richard; Veerkamp, Jacques H. (Dep.Chemicals with cas numbers 9007-28-7 and 2001-96-9 also play role. Biochem. Pulmonary Diseases, Univ. Nijmegen, Nijmegen, Neth.). American Journal of Respiratory Cell and Molecular Biology, 16(1), 75-84 (English) 1997 American Lung Association. CODEN: AJRBEL. ISSN: 1044-1549. DOCUMENT TYPE: Journal CA Section: 14 (Mammalian Pathological Biochemistry) The possible involvement of proteoglycans in the pathogenesis of emphysema was studied in rats by a single intratracheal instillation of p-nitrophenyl-b-D-xylopyranoside (b-D-xyloside), an inhibitor of proteoglycan synthesis. The first 3 days after instillation are characterized by mild hemorrhages, some infiltration of inflammatory cells, and edema. After 1 wk, lung morphol. was normal again. Forty days after instillation, considerable parenchymal destruction occurred as detd. by the mean linear intercept. Pulmonary fibrosis was not obsd. Instillation with p-nitrophenyl-a-D-xylopyranoside and p-nitrophenol did not induce parenchymal destruction, indicating the specificity of b-D-xyloside action. Urinary glycosaminoglycan (GAG) content of the b-D-xyloside-treated rats increased 15-fold during the first day after instillation, mainly due to elevated levels of chondroitin sulfate and dermatan sulfate. The increase was correlated to the extent of parenchymal destruction after 40 days. At day 2 and thereafter, levels were normal again. A short-term increase in dermatan and chondroitin sulfate content was also obsd. in serum, bronchoalveolar lavage (BAL) fluid, and lung tissue. Heparan sulfate content was decreased in BAL fluid and lung tissue. Instillation with p-nitrophenyl-a-D-xylopyranoside and p-nitrophenol did not induce an elevated GAG concn. in urine. Apparently, a disturbance in proteoglycan synthesis accompanied by an increase of (b-D-xyloside-primed) free GAGs results in loss of stability and integrity of the alveolar wall, leading to parenchymal destruction and emphysematous lesions. b-D-Xyloside treatment may be an alternative exptl. method for inducing emphysema. .

Study of the reaction of transglycosylation catalyzed by b-xylosidase from Aspergillus niger 15

Study of the reaction of transglycosylation catalyzed by b-xylosidase from Aspergillus niger 15. Tavobilov, I. M.; Maksimov, V.In this study， 2001-96-9 and 53362-87-1 are also used. I.; Rodionova, N. A. (A. N. Bakh Inst. Biochem., Moscow, USSR). Prikl. Biokhim. Mikrobiol., 20(1), 43-6 (Russian) 1984. CODEN: PBMIAK. ISSN: 0555-1099. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) The products of transglycosylation formed as a result of action of b-xylosidase from A. niger 15 on p-nitrophenyl-b-D-glucopyranoside and p-nitrophenyl-b-D-xylopyranoside were studied by chromatog. on Sephadex. They were formed at substrate concns. of 10-100 mM in amts. 7-10-fold less than those of hydrolysis products. Peculiarities of chromatog. of substrates, products of transglycosylation, and p-nitrophenol on Sephadex G-15 and G-25 were analyzed. .