Reference

Effect of polyamines on yeast cell-free protein synthesizing system

Effect of polyamines on yeast cell-free protein synthesizing system. I. Influence of spermine and spermidine on aminoacyl-tRNA transfer reaction. Wolska-Mitaszko, Barbara; Jakubowicz, Teresa; Gasior,Eugeniusz (Inst. Microbiol. Biochem., Univ. Marii Curie-Sklodowskiej, Lublin, Pol.Some commonly used reagents like 2018-61-3 and 306-67-2 are used in this experiment.). Acta Microbiol. Pol., 25(3), 187-97 (English) 1976. CODEN: AMPOAX. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) Spermine and spermidine added to a Saccharomyces cerevisiae cell-free protein synthesizing system increased the phenylalanine polymn. reaction several-fold at suboptimal concns. of Mg2+ and .apprx.2-fold at optimal amts. of Mg2+. The addn. of polyamines greatly stimulated the enzymic and nonenzymic binding of phenylalanyl-tRNA and N-acetylphenylalanyl-tRNA to ribosomes. The binding of the acetylated deriv. was higher than phenylalanyl-tRNA; however, the former was bound exclusively to the A site of the ribosome. Contrary to the binding process, the puromycin reaction was not stimulated by spermine added at a concn. which enhanced polyphenylalanine synthesis. One of the possible sites of polyamine action may be the binding of aminoacyl-tRNA to ribosomes. .

Tripeptides

Tripeptides.Some chemicals with cas registry numbers like 2018-61-3 and 2901-75-9 are also used. Molteni, Luigi; Vercesi, Gian P.; Antonini, Eraldo (Zambeletti, Dr. L., S.p.A., Italy). Ger. Offen. DE 2615129 28 Oct 1976, 25 pp. (German). (Germany). CODEN: GWXXBX. CLASS: IC: C07C103-52. PRIORITY: IT 75-22190 9 Apr 1975. DOCUMENT TYPE: Patent CA Section: 34 (Synthesis of Amino Acids, Peptides, and Proteins) Section cross-reference(s): 63 Ac-Phe-Phe-His-R (I, R = OH, OMe, NH2, NHMe) and the all-DL and L,D,L isomers of I (R = OMe) were prepd. as antiulcer agents. Thus, Ac-Phe-OH was coupled to H-Phe-OEt by dicyclohexylcarbodiimide (DCC) to give Ac-Phe-Phe-OEt which was sapond. and coupled to H-His-OMe by DCC to give I (R = OMe). The above tripeptides decreased the no. and strength of ulcers in rats. .