Reference

Rosmarinic acid from plant cell cultures

Rosmarinic acid from plant cell cultures. Ulbrich, Bernhard; Wiesner, Wilhelm; Arnes, Hans (Nattermann, A., und Cie. G.m.b.H., Fed. Rep. Ger.). Ger. Offen. DE 3247610 A1 5 Jul 1984, 11 pp. (German). (Germany). CODEN: GWXXBX. CLASS: IC: C07C059-90; A61K035-78; A61K031-19; C12P007-42. APPLICATION: DE 82-3247610 23 Dec 1982. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 11, 63 A suspension culture of Coleus blumei cells is reproduced in the maintenance medium of Gamborg et al. (1968) contg. 10-30 g sucrose [57-50-1]/L as molasses etc., and used for inoculation of prodn. medium with 1-10 g cells fresh wt./L, equiv. to 30 vol. parts of the maintenance culture/70 vol. parts prodn. medium. The prodn. medium contains 50 g sucrose/L and is fermented from 2 vols. of 75 g sucrose/L plus vol. used and reconditioned maintenance medium. The prodn. culture is aerated and agitated at 50 rpm with a patented stirrer which combines an intensive agitation with low shear for 7 days during which the content of rosmarinic acid [20283-92-5] increases at 0.4 g/L/day to a max. of 3.1 g/L at harvest, followed by lyophilization which yields a light-brown cell powder contg. 16-18 dry-wt.% rosmarinic acid. The lyophilization is used for manuf. of medical prepns. or for increasing the content of rosmarinic acid in herbal prepns.

Production of the secondary metabolite, rosmarinic acid, by plant cell suspension cultures

Production of the secondary metabolite, rosmarinic acid, by plant cell suspension cultures. Whitaker, Robert J.; Hashimoto, Takashi; Evans, David A. (DNA Plant Technol. Corp., Cinnaminson, NJ 08077, USA). Ann. N. Y. Acad. Sci., 435(Colloq. Biol. Sci., 1st, 1983), 364-6 (English) 1984. CODEN: ANYAA9. ISSN: 0077-8923. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 11 Rosmarinic acid (I) [20283-92-5] formation by intact leaves and suspension cultures of sage and Rosmarinus officinalis varieties was evaluated. In general, cultures produced markedly more I than intact leaves. Marked differences occurred in levels of chorismate mutase [9068-30-8] and phenylalanine ammonia-lyase [9024-28-6] between high- and low-producing cultures of Rosmarinus. The high-producing line exhibited major peaks of both enzymes at ~9-10 days which corresponded to the increase of I during this period, whereas the low-producing line was devoid of these peaks. Optimal levels of I for both lines were attained in the stationary growth phase (12-13 days).