Detail of "216667-08-2"

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Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by two-dimensional electrophoresis

All Rights Reserved. Assessment of four different detergents used to extract membrane proteins from Xylella fastidiosa by two-dimensional electrophoresis. Di Ciero, Luciana; de Bellato, Claudia M.; Meinhardt, Lyndel W.; Ferrari, Fernanda; Castellari, Rafael R.Chemicals with cas numbers 216667-08-2 and 15163-36-7 also play role.; Marangoni, Sergio; Novello, Jose C. (Departamento de Bioquimica, Universidade Estadual de Campinas, Campinas, SP, Brazil). Brazilian Journal of Microbiology, 35(3), 269-274 (English) 2004 Brazilian Society of Microbiology. CODEN: BJMRAH. ISSN: 1517-8382. DOCUMENT TYPE: Journal CA Section: 10 (Microbial, Algal, and Fungal Biochemistry) Section cross-reference(s): 9 Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to det. the optimal detergent for the solubilization of membrane proteins from the phytopathogenic bacterium Xylella fastidiosa. These proteins were differentially solubilized in distinct buffers contg. the detergent and subjected to bidimensional electrophoresis within the non-linear pH range of 3-10. The detergents ASB 14 and SB 3-10 were the most effective revealing 221 and 157 spots, resp. CHAPS and Triton X100 were less effective and revealed only 72 and 43 spots, resp. MALDI-TOF tryptic peptide mass fingerprinting of 18 excised proteins from the ASB 14 treatment revealed that 83% were membrane proteins and that the theor. efficiency of solubilization for ASB 14 was estd. to be 87%. This study demonstrates the effectiveness of the detergent ASB 14 for the solubilization of membrane proteins from the bacterium X. fastidiosa. .