Detail of "22144-77-0"

Reference

Action of cytochalasin E on polymorphonuclear leukocytes of guinea pig peritoneal exudates

Action of cytochalasin E on polymorphonuclear leukocytes of guinea pig peritoneal exudates. Nakagawara, A.; Shibata, Y.; Takeshige, K.; Minakami, S. (Sch. Med., Kyushu Univ., Fukuoka, Japan).There are some reagents with their cas registry numbers 14930-96-2 and 36011-19-5 are used in this study. Exp. Cell Res., 101(2), 225-34 (English) 1976. CODEN: ECREAL. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Actions of cytochalasins on polymorphonuclear leucocytes of guinea pig peritoneal exudates have been studied. When the leucocytes were treated with cytochalasin E (I) [36011-19-5], complete disappearance of pseudopods was obsd. during a time which would be related with the inhibition of particle ingestion. Intracellular granules migrated to the cell periphery in ectoplasma and were afterwards excluded from the cells. Later, formation of cytoplasmic protuberations with irregular shapes and variable diameters ("zeiosis") and of large vacuoles were obsd. Similar changes were also obsd. in monocytes. These morphol. changes were accompanied with metabolic alterations which mimicked those observed during phagocytosis: appearance of cyanide-insensitive respiration, stimulation of hexose monophosphate oxidative pathway, and release of superoxide anions and lysosomal enzymes into suspending medium. Other members of the cytochalasin family, cytochalasin C [22144-76-9] and D [22144-77-0], similarly induced release of superoxide anions, whereas cytochalasin A [14110-64-6] and B [14930-96-2] had no such effect on leucocytes. Cytochalasin E seemed to have dual effects on leucocytes, namely: (1) inhibition of cytokinesis which is a common effect of the cytochalasin family; and (2) triggering of metabolic changes and degranulation which are characteristics of phagocytotic process. .

Cytochalasin sensitive structures and lymphocyte activation

Cytochalasin sensitive structures and lymphocyte activation. Greene, W. C.; Parker, Christine M.; Parker, Ch. W. (Sch. Med., Washington Univ., St. Louis, Mo., USA). Exp. Cell Res., 103(1), 109-17 (English) 1976.In this article, certain chemicals are used. Some of their cas registry numbers are 60-92-4 and 36011-19-5 CODEN: ECREAL. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) In purified human peripheral blood lymphocytes, low (0.01-10 .mu.M) concns. of cytochalasins A [14110-64-6], B [14930-96-2], E [36011-19-5], and D [22144-77-0] produced marked augmentation of transport and metabolic responses to phytohemagglutinin (PHA) and concanavalin A (Con A) [11028-71-0], including effects on DNA synthesis, cyclic AMP [60-92-4] accumulation, phosphatidylinositol turnover, and sodium-dependent amino acid transport. At high concns. (10-100 .mu.M), these same responses were inhibited. Cytochalasin effects were minimal or absent if lectin was not present, indicating that these agents are acting by modulating the action of the lectin rather than through a direct effect on cell metab. By using [125I]ConA, the no. of lectin mols. bound per cell was shown not to be altered by the cytochalasins. Taken together with the previously reported effects of the cytochalasins on calcium uptake in lectin-stimulated lymphocytes, these observations suggest that microfilaments (or related cytochalasin-sensitive structures) play an important role in the modulation of lymphocyte activation. .